Split green fluorescent protein as a modular binding partner for protein crystallization

A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-­strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconsti...

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Detalles Bibliográficos
Autores principales: Nguyen, Hau B., Hung, Li-Wei, Yeates, Todd O., Terwilliger, Thomas C., Waldo, Geoffrey S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2013
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852656/
https://www.ncbi.nlm.nih.gov/pubmed/24311592
http://dx.doi.org/10.1107/S0907444913024608

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852656/
https://www.ncbi.nlm.nih.gov/pubmed/24311592
http://dx.doi.org/10.1107/S0907444913024608

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