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The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy

BACKGROUND: Determining the age of malaria vectors is essential for evaluating the impact of interventions that reduce the survival of wild mosquito populations and for estimating changes in vectorial capacity. Near infra-red spectroscopy (NIRS) is a simple and non-destructive method that has been u...

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Autores principales: Ntamatungiro, Alex J, Mayagaya, Valeriana S, Rieben, Stefan, Moore, Sarah J, Dowell, Floyd E, Maia, Marta F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852719/
https://www.ncbi.nlm.nih.gov/pubmed/24499515
http://dx.doi.org/10.1186/1756-3305-6-298
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author Ntamatungiro, Alex J
Mayagaya, Valeriana S
Rieben, Stefan
Moore, Sarah J
Dowell, Floyd E
Maia, Marta F
author_facet Ntamatungiro, Alex J
Mayagaya, Valeriana S
Rieben, Stefan
Moore, Sarah J
Dowell, Floyd E
Maia, Marta F
author_sort Ntamatungiro, Alex J
collection PubMed
description BACKGROUND: Determining the age of malaria vectors is essential for evaluating the impact of interventions that reduce the survival of wild mosquito populations and for estimating changes in vectorial capacity. Near infra-red spectroscopy (NIRS) is a simple and non-destructive method that has been used to determine the age and species of Anopheles gambiae s.l. by analyzing differences in absorption spectra. The spectra are affected by biochemical changes that occur during the life of a mosquito and could be influenced by senescence and also the life history of the mosquito, i.e., mating, blood feeding and egg-laying events. METHODS: To better understand these changes, we evaluated the influence of mosquito physiological status on NIR energy absorption spectra. Mosquitoes were kept in individual cups to permit record keeping of each individual insect’s life history. Mosquitoes of the same chronological age, but at different physiological stages, were scanned and compared using cross-validations. RESULTS: We observed a slight trend within some physiological stages that suggest older insects tend to be predicted as being physiologically more mature. It was advantageous to include mosquitoes of different chronological ages and physiological stages in calibrations, as it increases the robustness of the model resulting in better age predictions. CONCLUSIONS: Progression through different physiological statuses of An. arabiensis influences the chronological age prediction by the NIRS. Entomologists that wish to use NIR technology to predict the age of field-caught An. gambiae s.l from their study area should use a calibration developed from their field strain using mosquitoes of diverse chronological ages and physiological stages to increase the robustness and accuracy of the predictions.
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spelling pubmed-38527192013-12-06 The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy Ntamatungiro, Alex J Mayagaya, Valeriana S Rieben, Stefan Moore, Sarah J Dowell, Floyd E Maia, Marta F Parasit Vectors Research BACKGROUND: Determining the age of malaria vectors is essential for evaluating the impact of interventions that reduce the survival of wild mosquito populations and for estimating changes in vectorial capacity. Near infra-red spectroscopy (NIRS) is a simple and non-destructive method that has been used to determine the age and species of Anopheles gambiae s.l. by analyzing differences in absorption spectra. The spectra are affected by biochemical changes that occur during the life of a mosquito and could be influenced by senescence and also the life history of the mosquito, i.e., mating, blood feeding and egg-laying events. METHODS: To better understand these changes, we evaluated the influence of mosquito physiological status on NIR energy absorption spectra. Mosquitoes were kept in individual cups to permit record keeping of each individual insect’s life history. Mosquitoes of the same chronological age, but at different physiological stages, were scanned and compared using cross-validations. RESULTS: We observed a slight trend within some physiological stages that suggest older insects tend to be predicted as being physiologically more mature. It was advantageous to include mosquitoes of different chronological ages and physiological stages in calibrations, as it increases the robustness of the model resulting in better age predictions. CONCLUSIONS: Progression through different physiological statuses of An. arabiensis influences the chronological age prediction by the NIRS. Entomologists that wish to use NIR technology to predict the age of field-caught An. gambiae s.l from their study area should use a calibration developed from their field strain using mosquitoes of diverse chronological ages and physiological stages to increase the robustness and accuracy of the predictions. BioMed Central 2013-10-14 /pmc/articles/PMC3852719/ /pubmed/24499515 http://dx.doi.org/10.1186/1756-3305-6-298 Text en Copyright © 2013 Ntamatungiro et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ntamatungiro, Alex J
Mayagaya, Valeriana S
Rieben, Stefan
Moore, Sarah J
Dowell, Floyd E
Maia, Marta F
The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy
title The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy
title_full The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy
title_fullStr The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy
title_full_unstemmed The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy
title_short The influence of physiological status on age prediction of Anopheles arabiensis using near infra-red spectroscopy
title_sort influence of physiological status on age prediction of anopheles arabiensis using near infra-red spectroscopy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852719/
https://www.ncbi.nlm.nih.gov/pubmed/24499515
http://dx.doi.org/10.1186/1756-3305-6-298
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