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Empirical assessment of sequencing errors for high throughput pyrosequencing data

BACKGROUND: Sequencing-by-synthesis technologies significantly improve over the Sanger method in terms of speed and cost per base. However, they still usually fail to compete in terms of read length and quality. Current high-throughput implementations of the pyrosequencing technique yield reads whos...

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Detalles Bibliográficos
Autores principales: da Fonseca, Paulo GS, Paiva, Jorge AP, Almeida, Luiz GP, Vasconcelos, Ana TR, Freitas, Ana T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852801/
https://www.ncbi.nlm.nih.gov/pubmed/23339526
http://dx.doi.org/10.1186/1756-0500-6-25

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