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Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
BACKGROUND: Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign follicular thyroid adenoma (FTA) is a great challenge for even an experienced pathologist and requires special effort. Molecular markers may potentially support a differential diagnosis between FTC and FT...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852913/ https://www.ncbi.nlm.nih.gov/pubmed/24099521 http://dx.doi.org/10.1186/1755-8794-6-38 |
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author | Pfeifer, Aleksandra Wojtas, Bartosz Oczko-Wojciechowska, Malgorzata Kukulska, Aleksandra Czarniecka, Agnieszka Eszlinger, Markus Musholt, Thomas Stokowy, Tomasz Swierniak, Michal Stobiecka, Ewa Rusinek, Dagmara Tyszkiewicz, Tomasz Kowal, Monika Jarzab, Michal Hauptmann, Steffen Lange, Dariusz Paschke, Ralf Jarzab, Barbara |
author_facet | Pfeifer, Aleksandra Wojtas, Bartosz Oczko-Wojciechowska, Malgorzata Kukulska, Aleksandra Czarniecka, Agnieszka Eszlinger, Markus Musholt, Thomas Stokowy, Tomasz Swierniak, Michal Stobiecka, Ewa Rusinek, Dagmara Tyszkiewicz, Tomasz Kowal, Monika Jarzab, Michal Hauptmann, Steffen Lange, Dariusz Paschke, Ralf Jarzab, Barbara |
author_sort | Pfeifer, Aleksandra |
collection | PubMed |
description | BACKGROUND: Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign follicular thyroid adenoma (FTA) is a great challenge for even an experienced pathologist and requires special effort. Molecular markers may potentially support a differential diagnosis between FTC and FTA in postoperative specimens. The purpose of this study was to derive molecular support for differential post-operative diagnosis, in the form of a simple multigene mRNA-based classifier that would differentiate between FTC and FTA tissue samples. METHODS: A molecular classifier was created based on a combined analysis of two microarray datasets (using 66 thyroid samples). The performance of the classifier was assessed using an independent dataset comprising 71 formalin-fixed paraffin-embedded (FFPE) samples (31 FTC and 40 FTA), which were analysed by quantitative real-time PCR (qPCR). In addition, three other microarray datasets (62 samples) were used to confirm the utility of the classifier. RESULTS: Five of 8 genes selected from training datasets (ELMO1, EMCN, ITIH5, KCNAB1, SLCO2A1) were amplified by qPCR in FFPE material from an independent sample set. Three other genes did not amplify in FFPE material, probably due to low abundance. All 5 analysed genes were downregulated in FTC compared to FTA. The sensitivity and specificity of the 5-gene classifier tested on the FFPE dataset were 71% and 72%, respectively. CONCLUSIONS: The proposed approach could support histopathological examination: 5-gene classifier may aid in molecular discrimination between FTC and FTA in FFPE material. |
format | Online Article Text |
id | pubmed-3852913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38529132013-12-07 Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues Pfeifer, Aleksandra Wojtas, Bartosz Oczko-Wojciechowska, Malgorzata Kukulska, Aleksandra Czarniecka, Agnieszka Eszlinger, Markus Musholt, Thomas Stokowy, Tomasz Swierniak, Michal Stobiecka, Ewa Rusinek, Dagmara Tyszkiewicz, Tomasz Kowal, Monika Jarzab, Michal Hauptmann, Steffen Lange, Dariusz Paschke, Ralf Jarzab, Barbara BMC Med Genomics Research Article BACKGROUND: Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign follicular thyroid adenoma (FTA) is a great challenge for even an experienced pathologist and requires special effort. Molecular markers may potentially support a differential diagnosis between FTC and FTA in postoperative specimens. The purpose of this study was to derive molecular support for differential post-operative diagnosis, in the form of a simple multigene mRNA-based classifier that would differentiate between FTC and FTA tissue samples. METHODS: A molecular classifier was created based on a combined analysis of two microarray datasets (using 66 thyroid samples). The performance of the classifier was assessed using an independent dataset comprising 71 formalin-fixed paraffin-embedded (FFPE) samples (31 FTC and 40 FTA), which were analysed by quantitative real-time PCR (qPCR). In addition, three other microarray datasets (62 samples) were used to confirm the utility of the classifier. RESULTS: Five of 8 genes selected from training datasets (ELMO1, EMCN, ITIH5, KCNAB1, SLCO2A1) were amplified by qPCR in FFPE material from an independent sample set. Three other genes did not amplify in FFPE material, probably due to low abundance. All 5 analysed genes were downregulated in FTC compared to FTA. The sensitivity and specificity of the 5-gene classifier tested on the FFPE dataset were 71% and 72%, respectively. CONCLUSIONS: The proposed approach could support histopathological examination: 5-gene classifier may aid in molecular discrimination between FTC and FTA in FFPE material. BioMed Central 2013-10-07 /pmc/articles/PMC3852913/ /pubmed/24099521 http://dx.doi.org/10.1186/1755-8794-6-38 Text en Copyright © 2013 Pfeifer et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pfeifer, Aleksandra Wojtas, Bartosz Oczko-Wojciechowska, Malgorzata Kukulska, Aleksandra Czarniecka, Agnieszka Eszlinger, Markus Musholt, Thomas Stokowy, Tomasz Swierniak, Michal Stobiecka, Ewa Rusinek, Dagmara Tyszkiewicz, Tomasz Kowal, Monika Jarzab, Michal Hauptmann, Steffen Lange, Dariusz Paschke, Ralf Jarzab, Barbara Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
title | Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
title_full | Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
title_fullStr | Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
title_full_unstemmed | Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
title_short | Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
title_sort | molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852913/ https://www.ncbi.nlm.nih.gov/pubmed/24099521 http://dx.doi.org/10.1186/1755-8794-6-38 |
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