Cargando…

Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues

BACKGROUND: Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign follicular thyroid adenoma (FTA) is a great challenge for even an experienced pathologist and requires special effort. Molecular markers may potentially support a differential diagnosis between FTC and FT...

Descripción completa

Detalles Bibliográficos
Autores principales: Pfeifer, Aleksandra, Wojtas, Bartosz, Oczko-Wojciechowska, Malgorzata, Kukulska, Aleksandra, Czarniecka, Agnieszka, Eszlinger, Markus, Musholt, Thomas, Stokowy, Tomasz, Swierniak, Michal, Stobiecka, Ewa, Rusinek, Dagmara, Tyszkiewicz, Tomasz, Kowal, Monika, Jarzab, Michal, Hauptmann, Steffen, Lange, Dariusz, Paschke, Ralf, Jarzab, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852913/
https://www.ncbi.nlm.nih.gov/pubmed/24099521
http://dx.doi.org/10.1186/1755-8794-6-38
_version_ 1782478744345116672
author Pfeifer, Aleksandra
Wojtas, Bartosz
Oczko-Wojciechowska, Malgorzata
Kukulska, Aleksandra
Czarniecka, Agnieszka
Eszlinger, Markus
Musholt, Thomas
Stokowy, Tomasz
Swierniak, Michal
Stobiecka, Ewa
Rusinek, Dagmara
Tyszkiewicz, Tomasz
Kowal, Monika
Jarzab, Michal
Hauptmann, Steffen
Lange, Dariusz
Paschke, Ralf
Jarzab, Barbara
author_facet Pfeifer, Aleksandra
Wojtas, Bartosz
Oczko-Wojciechowska, Malgorzata
Kukulska, Aleksandra
Czarniecka, Agnieszka
Eszlinger, Markus
Musholt, Thomas
Stokowy, Tomasz
Swierniak, Michal
Stobiecka, Ewa
Rusinek, Dagmara
Tyszkiewicz, Tomasz
Kowal, Monika
Jarzab, Michal
Hauptmann, Steffen
Lange, Dariusz
Paschke, Ralf
Jarzab, Barbara
author_sort Pfeifer, Aleksandra
collection PubMed
description BACKGROUND: Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign follicular thyroid adenoma (FTA) is a great challenge for even an experienced pathologist and requires special effort. Molecular markers may potentially support a differential diagnosis between FTC and FTA in postoperative specimens. The purpose of this study was to derive molecular support for differential post-operative diagnosis, in the form of a simple multigene mRNA-based classifier that would differentiate between FTC and FTA tissue samples. METHODS: A molecular classifier was created based on a combined analysis of two microarray datasets (using 66 thyroid samples). The performance of the classifier was assessed using an independent dataset comprising 71 formalin-fixed paraffin-embedded (FFPE) samples (31 FTC and 40 FTA), which were analysed by quantitative real-time PCR (qPCR). In addition, three other microarray datasets (62 samples) were used to confirm the utility of the classifier. RESULTS: Five of 8 genes selected from training datasets (ELMO1, EMCN, ITIH5, KCNAB1, SLCO2A1) were amplified by qPCR in FFPE material from an independent sample set. Three other genes did not amplify in FFPE material, probably due to low abundance. All 5 analysed genes were downregulated in FTC compared to FTA. The sensitivity and specificity of the 5-gene classifier tested on the FFPE dataset were 71% and 72%, respectively. CONCLUSIONS: The proposed approach could support histopathological examination: 5-gene classifier may aid in molecular discrimination between FTC and FTA in FFPE material.
format Online
Article
Text
id pubmed-3852913
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-38529132013-12-07 Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues Pfeifer, Aleksandra Wojtas, Bartosz Oczko-Wojciechowska, Malgorzata Kukulska, Aleksandra Czarniecka, Agnieszka Eszlinger, Markus Musholt, Thomas Stokowy, Tomasz Swierniak, Michal Stobiecka, Ewa Rusinek, Dagmara Tyszkiewicz, Tomasz Kowal, Monika Jarzab, Michal Hauptmann, Steffen Lange, Dariusz Paschke, Ralf Jarzab, Barbara BMC Med Genomics Research Article BACKGROUND: Differential diagnosis between malignant follicular thyroid cancer (FTC) and benign follicular thyroid adenoma (FTA) is a great challenge for even an experienced pathologist and requires special effort. Molecular markers may potentially support a differential diagnosis between FTC and FTA in postoperative specimens. The purpose of this study was to derive molecular support for differential post-operative diagnosis, in the form of a simple multigene mRNA-based classifier that would differentiate between FTC and FTA tissue samples. METHODS: A molecular classifier was created based on a combined analysis of two microarray datasets (using 66 thyroid samples). The performance of the classifier was assessed using an independent dataset comprising 71 formalin-fixed paraffin-embedded (FFPE) samples (31 FTC and 40 FTA), which were analysed by quantitative real-time PCR (qPCR). In addition, three other microarray datasets (62 samples) were used to confirm the utility of the classifier. RESULTS: Five of 8 genes selected from training datasets (ELMO1, EMCN, ITIH5, KCNAB1, SLCO2A1) were amplified by qPCR in FFPE material from an independent sample set. Three other genes did not amplify in FFPE material, probably due to low abundance. All 5 analysed genes were downregulated in FTC compared to FTA. The sensitivity and specificity of the 5-gene classifier tested on the FFPE dataset were 71% and 72%, respectively. CONCLUSIONS: The proposed approach could support histopathological examination: 5-gene classifier may aid in molecular discrimination between FTC and FTA in FFPE material. BioMed Central 2013-10-07 /pmc/articles/PMC3852913/ /pubmed/24099521 http://dx.doi.org/10.1186/1755-8794-6-38 Text en Copyright © 2013 Pfeifer et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pfeifer, Aleksandra
Wojtas, Bartosz
Oczko-Wojciechowska, Malgorzata
Kukulska, Aleksandra
Czarniecka, Agnieszka
Eszlinger, Markus
Musholt, Thomas
Stokowy, Tomasz
Swierniak, Michal
Stobiecka, Ewa
Rusinek, Dagmara
Tyszkiewicz, Tomasz
Kowal, Monika
Jarzab, Michal
Hauptmann, Steffen
Lange, Dariusz
Paschke, Ralf
Jarzab, Barbara
Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
title Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
title_full Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
title_fullStr Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
title_full_unstemmed Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
title_short Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
title_sort molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852913/
https://www.ncbi.nlm.nih.gov/pubmed/24099521
http://dx.doi.org/10.1186/1755-8794-6-38
work_keys_str_mv AT pfeiferaleksandra moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT wojtasbartosz moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT oczkowojciechowskamalgorzata moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT kukulskaaleksandra moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT czarnieckaagnieszka moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT eszlingermarkus moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT musholtthomas moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT stokowytomasz moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT swierniakmichal moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT stobieckaewa moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT rusinekdagmara moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT tyszkiewicztomasz moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT kowalmonika moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT jarzabmichal moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT hauptmannsteffen moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT langedariusz moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT paschkeralf moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues
AT jarzabbarbara moleculardifferentialdiagnosisoffollicularthyroidcarcinomaandadenomabasedongeneexpressionprofilingbyusingformalinfixedparaffinembeddedtissues