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The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation

BACKGROUND: The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ven...

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Autores principales: Pu, Qin, Abduelmula, Aisha, Masyuk, Maryna, Theiss, Carsten, Schwandulla, Dieter, Hans, Michael, Patel, Ketan, Brand-Saberi, Beate, Huang, Ruijin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853214/
https://www.ncbi.nlm.nih.gov/pubmed/24138189
http://dx.doi.org/10.1186/1471-213X-13-37
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author Pu, Qin
Abduelmula, Aisha
Masyuk, Maryna
Theiss, Carsten
Schwandulla, Dieter
Hans, Michael
Patel, Ketan
Brand-Saberi, Beate
Huang, Ruijin
author_facet Pu, Qin
Abduelmula, Aisha
Masyuk, Maryna
Theiss, Carsten
Schwandulla, Dieter
Hans, Michael
Patel, Ketan
Brand-Saberi, Beate
Huang, Ruijin
author_sort Pu, Qin
collection PubMed
description BACKGROUND: The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally. RESULTS: Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome. CONCLUSION: Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome.
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spelling pubmed-38532142013-12-07 The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation Pu, Qin Abduelmula, Aisha Masyuk, Maryna Theiss, Carsten Schwandulla, Dieter Hans, Michael Patel, Ketan Brand-Saberi, Beate Huang, Ruijin BMC Dev Biol Research Article BACKGROUND: The myotome is the primitive skeletal muscle that forms within the embryonic metameric body wall. It can be subdivided into an epaxial and hypaxial domain. It has been shown that the formation of the epaxial myotome requires the dorsomedial lip of the dermomyotome (DML). Although the ventrolateral lip (VLL) of the dermomyotome is believed to be required for the formation of the hypaxial myotome, experimentally evidence for this statement still needs to be provided. Provision of such data would enable the resolution of a debate regarding the formation of the hypaxial dermomyotome. Two mechanisms have been proposed for this tissue. The first proposes that the intermediate dermomyotome undergoes cellular expansion thereby pushing the ventral lateral lip in a lateral direction (translocation). In contrast, the alternative view holds that the ventral lateral lip grows laterally. RESULTS: Using time lapse confocal microscopy, we observed that the GFP-labelled ventrolateral lip (VLL) of the dermomyotome grows rather than translocates in a lateral direction. The necessity of the VLL for lateral extension of the myotome was addressed by ablation studies. We found that the hypaxial myotome did not form after VLL ablation. In contrast, the removal of an intermediate portion of the dermomyotome had very little effect of the hypaxial myotome. These results demonstrate that the VLL is required for the formation of the hypaxial myotome. CONCLUSION: Our study demonstrates that the dermomyotome ventrolateral lip is essential for the hypaxial myotome formation and supports the lip extension model. Therefore, despite being under independent signalling controls, both the dorsomedial and ventrolateral lip fulfil the same function, i.e. they extend into adjacent regions permitting the growth of the myotome. BioMed Central 2013-10-18 /pmc/articles/PMC3853214/ /pubmed/24138189 http://dx.doi.org/10.1186/1471-213X-13-37 Text en Copyright © 2013 Pu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pu, Qin
Abduelmula, Aisha
Masyuk, Maryna
Theiss, Carsten
Schwandulla, Dieter
Hans, Michael
Patel, Ketan
Brand-Saberi, Beate
Huang, Ruijin
The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
title The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
title_full The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
title_fullStr The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
title_full_unstemmed The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
title_short The dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
title_sort dermomyotome ventrolateral lip is essential for the hypaxial myotome formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853214/
https://www.ncbi.nlm.nih.gov/pubmed/24138189
http://dx.doi.org/10.1186/1471-213X-13-37
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