Cargando…
Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma
BACKGROUND: Although epidermal growth factor receptor (EGFR) inhibitor treatment showed modest response in several clinical trials in esophageal squamous cell carcinoma (ESCC) patients, it has been reported that the frequency of EGFR mutations varied largely. The aim of this study was to investigate...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853380/ https://www.ncbi.nlm.nih.gov/pubmed/24103528 http://dx.doi.org/10.1186/1477-7819-11-266 |
_version_ | 1782294623899615232 |
---|---|
author | Cui, Yong Chang, Dong Liu, Mingliang Lin, Changjin Zhao, Baojian Zhang, Xu Gong, Min |
author_facet | Cui, Yong Chang, Dong Liu, Mingliang Lin, Changjin Zhao, Baojian Zhang, Xu Gong, Min |
author_sort | Cui, Yong |
collection | PubMed |
description | BACKGROUND: Although epidermal growth factor receptor (EGFR) inhibitor treatment showed modest response in several clinical trials in esophageal squamous cell carcinoma (ESCC) patients, it has been reported that the frequency of EGFR mutations varied largely. The aim of this study was to investigate the existence of EGFR mutations in Chinese esophageal squamous cell carcinomas. METHODS: Formalin-fixed paraffin-embedded surgically resected tumor samples were obtained from 127 randomly selected Chinese patients with ESCC. The most common EGFR mutations, including in-frame deletions in exon 19 and base substitutions in exon 21, were detected by denaturing high performance liquid chromatography (DHPLC) and direct sequencing simultaneously. K-RAS mutations in codons 12 and 13 were detected by direct sequencing. RESULTS: In this study, L858R missense mutations of the EGFR gene were found in 8 out of 127 patients (6.3%) by DHPLC but no mutation was observed by direct sequencing. In addition, K-RAS mutation was detected in 2 out of 127 (1.6%) patients by direct sequencing. CONCLUSIONS: The incidence of EGFR mutations was relatively high using DHPLC method but no mutation with direct sequencing in Chinese ESCC patients. |
format | Online Article Text |
id | pubmed-3853380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38533802013-12-07 Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma Cui, Yong Chang, Dong Liu, Mingliang Lin, Changjin Zhao, Baojian Zhang, Xu Gong, Min World J Surg Oncol Technical Innovations BACKGROUND: Although epidermal growth factor receptor (EGFR) inhibitor treatment showed modest response in several clinical trials in esophageal squamous cell carcinoma (ESCC) patients, it has been reported that the frequency of EGFR mutations varied largely. The aim of this study was to investigate the existence of EGFR mutations in Chinese esophageal squamous cell carcinomas. METHODS: Formalin-fixed paraffin-embedded surgically resected tumor samples were obtained from 127 randomly selected Chinese patients with ESCC. The most common EGFR mutations, including in-frame deletions in exon 19 and base substitutions in exon 21, were detected by denaturing high performance liquid chromatography (DHPLC) and direct sequencing simultaneously. K-RAS mutations in codons 12 and 13 were detected by direct sequencing. RESULTS: In this study, L858R missense mutations of the EGFR gene were found in 8 out of 127 patients (6.3%) by DHPLC but no mutation was observed by direct sequencing. In addition, K-RAS mutation was detected in 2 out of 127 (1.6%) patients by direct sequencing. CONCLUSIONS: The incidence of EGFR mutations was relatively high using DHPLC method but no mutation with direct sequencing in Chinese ESCC patients. BioMed Central 2013-10-09 /pmc/articles/PMC3853380/ /pubmed/24103528 http://dx.doi.org/10.1186/1477-7819-11-266 Text en Copyright © 2013 Cui et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Technical Innovations Cui, Yong Chang, Dong Liu, Mingliang Lin, Changjin Zhao, Baojian Zhang, Xu Gong, Min Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma |
title | Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma |
title_full | Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma |
title_fullStr | Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma |
title_full_unstemmed | Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma |
title_short | Identification of exon 19 and 21 mutations of EGFR gene in Chinese patients with esophageal squamous cell carcinoma |
title_sort | identification of exon 19 and 21 mutations of egfr gene in chinese patients with esophageal squamous cell carcinoma |
topic | Technical Innovations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853380/ https://www.ncbi.nlm.nih.gov/pubmed/24103528 http://dx.doi.org/10.1186/1477-7819-11-266 |
work_keys_str_mv | AT cuiyong identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma AT changdong identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma AT liumingliang identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma AT linchangjin identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma AT zhaobaojian identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma AT zhangxu identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma AT gongmin identificationofexon19and21mutationsofegfrgeneinchinesepatientswithesophagealsquamouscellcarcinoma |