Cargando…

Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla

ABSTRACTS: BACKGROUND: The aims of this study were to evaluate the antidiabetic activity and to detect molecular size of Pseudostellaria heterophylla polysaccharide (PHP). Pseudostellaria heterophylla is a medicine extensively used in traditional Chinese medicine formulas to treat diabetes and its c...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Juan, Pang, Wensheng, chen, Jinlong, Bai, Shaowei, Zheng, Zhenzhu, Wu, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853478/
https://www.ncbi.nlm.nih.gov/pubmed/24131482
http://dx.doi.org/10.1186/1472-6882-13-267
_version_ 1782294642275909632
author Hu, Juan
Pang, Wensheng
chen, Jinlong
Bai, Shaowei
Zheng, Zhenzhu
Wu, Xiaohua
author_facet Hu, Juan
Pang, Wensheng
chen, Jinlong
Bai, Shaowei
Zheng, Zhenzhu
Wu, Xiaohua
author_sort Hu, Juan
collection PubMed
description ABSTRACTS: BACKGROUND: The aims of this study were to evaluate the antidiabetic activity and to detect molecular size of Pseudostellaria heterophylla polysaccharide (PHP). Pseudostellaria heterophylla is a medicine extensively used in traditional Chinese medicine formulas to treat diabetes and its complications. METHODS: Molecular weight of PHP was determined by gel permeation chromatography combined with phenol-sulphuric acid method and the monosaccharides composition was determined by HPLC with a precolumn derivatization. Four polysaccharides with different molecular weight were compared for hypoglycemic active on two animal models both high does alloxan induced type1 diabetic mellitus (T1DM) and high-fat/lower does streptozotocin induced type2 diabetic mellitus (T2DM). Blood sugar, glucose tolerance, and insulin tolerance were detected. Rat serum IL-1β, IL-2, IL-10, Leptin, TNF-α, Acrp30 and CRP were also analyzed by sandwich-ELISA approaches to preliminary probe the hypoglycemic mechanism of PHP. RESULTS: The hypoglycemic effects related to molecular size of polysaccharide were more effective against T2DM than T1DM. PHP comprise four monosaccharides of galacturonic acid, glucose, galactose and arabinos. T2DM rats daily receiving oral dose of polysaccharide(100 ~ 400 mg/kg) with 50 ~ 210 kDa molecular weight (PF40) could not only significantly lower blood sugar but also reduce total triglyceride level in serum. PF40 improves in insulin tolerance inhibited the expression of some biomarkers including inflammatory cytokine TNF-α and elevated anti-inflammatory cytokine IL-10, regulated adiponectin Acrp30 and leptin. CONCLUSIONS: PF40 prevent the cascade of inflammatory events in the treatment of T2DM to block overweight progresses to obesity.
format Online
Article
Text
id pubmed-3853478
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-38534782013-12-07 Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla Hu, Juan Pang, Wensheng chen, Jinlong Bai, Shaowei Zheng, Zhenzhu Wu, Xiaohua BMC Complement Altern Med Research Article ABSTRACTS: BACKGROUND: The aims of this study were to evaluate the antidiabetic activity and to detect molecular size of Pseudostellaria heterophylla polysaccharide (PHP). Pseudostellaria heterophylla is a medicine extensively used in traditional Chinese medicine formulas to treat diabetes and its complications. METHODS: Molecular weight of PHP was determined by gel permeation chromatography combined with phenol-sulphuric acid method and the monosaccharides composition was determined by HPLC with a precolumn derivatization. Four polysaccharides with different molecular weight were compared for hypoglycemic active on two animal models both high does alloxan induced type1 diabetic mellitus (T1DM) and high-fat/lower does streptozotocin induced type2 diabetic mellitus (T2DM). Blood sugar, glucose tolerance, and insulin tolerance were detected. Rat serum IL-1β, IL-2, IL-10, Leptin, TNF-α, Acrp30 and CRP were also analyzed by sandwich-ELISA approaches to preliminary probe the hypoglycemic mechanism of PHP. RESULTS: The hypoglycemic effects related to molecular size of polysaccharide were more effective against T2DM than T1DM. PHP comprise four monosaccharides of galacturonic acid, glucose, galactose and arabinos. T2DM rats daily receiving oral dose of polysaccharide(100 ~ 400 mg/kg) with 50 ~ 210 kDa molecular weight (PF40) could not only significantly lower blood sugar but also reduce total triglyceride level in serum. PF40 improves in insulin tolerance inhibited the expression of some biomarkers including inflammatory cytokine TNF-α and elevated anti-inflammatory cytokine IL-10, regulated adiponectin Acrp30 and leptin. CONCLUSIONS: PF40 prevent the cascade of inflammatory events in the treatment of T2DM to block overweight progresses to obesity. BioMed Central 2013-10-16 /pmc/articles/PMC3853478/ /pubmed/24131482 http://dx.doi.org/10.1186/1472-6882-13-267 Text en Copyright © 2013 Hu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hu, Juan
Pang, Wensheng
chen, Jinlong
Bai, Shaowei
Zheng, Zhenzhu
Wu, Xiaohua
Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla
title Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla
title_full Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla
title_fullStr Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla
title_full_unstemmed Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla
title_short Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla
title_sort hypoglycemic effect of polysaccharides with different molecular weight of pseudostellaria heterophylla
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853478/
https://www.ncbi.nlm.nih.gov/pubmed/24131482
http://dx.doi.org/10.1186/1472-6882-13-267
work_keys_str_mv AT hujuan hypoglycemiceffectofpolysaccharideswithdifferentmolecularweightofpseudostellariaheterophylla
AT pangwensheng hypoglycemiceffectofpolysaccharideswithdifferentmolecularweightofpseudostellariaheterophylla
AT chenjinlong hypoglycemiceffectofpolysaccharideswithdifferentmolecularweightofpseudostellariaheterophylla
AT baishaowei hypoglycemiceffectofpolysaccharideswithdifferentmolecularweightofpseudostellariaheterophylla
AT zhengzhenzhu hypoglycemiceffectofpolysaccharideswithdifferentmolecularweightofpseudostellariaheterophylla
AT wuxiaohua hypoglycemiceffectofpolysaccharideswithdifferentmolecularweightofpseudostellariaheterophylla