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Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats

Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rat...

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Autores principales: Rodrigues, L.P., Iglesias, D., Nicola, F.C., Steffens, D., Valentim, L., Witczak, A., Zanatta, G., Achaval, M., Pranke, P., Netto, C.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854143/
https://www.ncbi.nlm.nih.gov/pubmed/22183246
http://dx.doi.org/10.1590/S0100-879X2011007500162
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author Rodrigues, L.P.
Iglesias, D.
Nicola, F.C.
Steffens, D.
Valentim, L.
Witczak, A.
Zanatta, G.
Achaval, M.
Pranke, P.
Netto, C.A.
author_facet Rodrigues, L.P.
Iglesias, D.
Nicola, F.C.
Steffens, D.
Valentim, L.
Witczak, A.
Zanatta, G.
Achaval, M.
Pranke, P.
Netto, C.A.
author_sort Rodrigues, L.P.
collection PubMed
description Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 10(6) cells diluted in 10 µL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at a concentration of 5 x 10(6) cells diluted in 150 µL 0.9% NaCl (N = 12-14 per group). The transplanted animals were immunosuppressed with cyclosporin-A (10 mg/kg per day). The BBB scale was used to evaluate motor behavior and the injury site was analyzed with immunofluorescent markers to label human transplanted cells, oligodendrocytes, neurons, and astrocytes. Spinal cord injury rats had 25% loss of cord tissue and cell treatment did not affect lesion extension. Transplanted cells survived in the injured area for 6 weeks after the procedure and both transplanted groups showed better motor recovery than the untreated ones (P < 0.05). The transplantation of mononuclear cells from human umbilical cord blood promoted functional recovery with no evidence of cell differentiation.
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spelling pubmed-38541432013-12-16 Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats Rodrigues, L.P. Iglesias, D. Nicola, F.C. Steffens, D. Valentim, L. Witczak, A. Zanatta, G. Achaval, M. Pranke, P. Netto, C.A. Braz J Med Biol Res Short Communication Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 10(6) cells diluted in 10 µL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at a concentration of 5 x 10(6) cells diluted in 150 µL 0.9% NaCl (N = 12-14 per group). The transplanted animals were immunosuppressed with cyclosporin-A (10 mg/kg per day). The BBB scale was used to evaluate motor behavior and the injury site was analyzed with immunofluorescent markers to label human transplanted cells, oligodendrocytes, neurons, and astrocytes. Spinal cord injury rats had 25% loss of cord tissue and cell treatment did not affect lesion extension. Transplanted cells survived in the injured area for 6 weeks after the procedure and both transplanted groups showed better motor recovery than the untreated ones (P < 0.05). The transplantation of mononuclear cells from human umbilical cord blood promoted functional recovery with no evidence of cell differentiation. Sociedade Brasileira de Medicina Tropical 2011-12-23 /pmc/articles/PMC3854143/ /pubmed/22183246 http://dx.doi.org/10.1590/S0100-879X2011007500162 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Rodrigues, L.P.
Iglesias, D.
Nicola, F.C.
Steffens, D.
Valentim, L.
Witczak, A.
Zanatta, G.
Achaval, M.
Pranke, P.
Netto, C.A.
Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
title Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
title_full Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
title_fullStr Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
title_full_unstemmed Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
title_short Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
title_sort transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in wistar rats
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854143/
https://www.ncbi.nlm.nih.gov/pubmed/22183246
http://dx.doi.org/10.1590/S0100-879X2011007500162
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