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The role of Ca(2+)/calmodulin-dependent protein kinase II and calcineurin in TNF-α-induced myocardial hypertrophy

We investigated whether Ca(2+)/calmodulin-dependent kinase II (CaMKII) and calcineurin (CaN) are involved in myocardial hypertrophy induced by tumor necrosis factor α (TNF-α). The cardiomyocytes of neonatal Wistar rats (1-2 days old) were cultured and stimulated by TNF-α (100 µg/L), and Ca(2+) signa...

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Detalles Bibliográficos
Autores principales: Wang, Gui-Jun, Wang, Hong-Xin, Yao, Yu-Sheng, Guo, Lian-Yi, Liu, Pei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854156/
https://www.ncbi.nlm.nih.gov/pubmed/22832601
http://dx.doi.org/10.1590/S0100-879X2012007500121
Descripción
Sumario:We investigated whether Ca(2+)/calmodulin-dependent kinase II (CaMKII) and calcineurin (CaN) are involved in myocardial hypertrophy induced by tumor necrosis factor α (TNF-α). The cardiomyocytes of neonatal Wistar rats (1-2 days old) were cultured and stimulated by TNF-α (100 µg/L), and Ca(2+) signal transduction was blocked by several antagonists, including BAPTA (4 µM), KN-93 (0.2 µM) and cyclosporin A (CsA, 0.2 µM). Protein content, protein synthesis, cardiomyocyte volumes, [Ca(2+)](i) transients, CaMKIIδ(B) and CaN were evaluated by the Lowry method, [(3)H]-leucine incorporation, a computerized image analysis system, a Till imaging system, and Western blot analysis, respectively. TNF-α induced a significant increase in protein content in a dose-dependent manner from 10 µg/L (53.56 µg protein/well) to 100 µg/L (72.18 µg protein/well), and in a time-dependent manner from 12 h (37.42 µg protein/well) to 72 h (42.81 µg protein/well). TNF-α (100 µg/L) significantly increased the amplitude of spontaneous [Ca(2+)](i) transients, the total protein content, cell size, and [(3)H]-leucine incorporation in cultured cardiomyocytes, which was abolished by 4 µM BAPTA, an intracellular Ca(2+) chelator. The increases in protein content, cell size and [(3)H]-leucine incorporation were abolished by 0.2 µM KN-93 or 0.2 µM CsA. TNF-α increased the expression of CaMKIIδ(B) by 35.21% and that of CaN by 22.22% compared to control. These effects were abolished by 4 µM BAPTA, which itself had no effect. These results suggest that TNF-α induces increases in [Ca(2+)](i), CaMKIIδ(B) and CaN and promotes cardiac hypertrophy. Therefore, we hypothesize that the Ca(2+)/CaMKII- and CaN-dependent signaling pathways are involved in myocardial hypertrophy induced by TNF-α.