Cargando…

Negative correlation between phospholipase and esterase activity produced by Fusarium isolates

Fusarium species have emerged as one of the more outstanding groups of clinically important filamentous fungi, causing localized and life-threatening invasive infections with high morbidity and mortality. The ability to produce different types of hydrolytic enzymes is thought to be an important viru...

Descripción completa

Detalles Bibliográficos
Autores principales: Ishida, K., Alviano, D.S., Silva, B.G., Guerra, C.R., Costa, A.S., Nucci, M., Alviano, C.S., Rozental, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854292/
https://www.ncbi.nlm.nih.gov/pubmed/22415116
http://dx.doi.org/10.1590/S0100-879X2012007500034
_version_ 1782294775116857344
author Ishida, K.
Alviano, D.S.
Silva, B.G.
Guerra, C.R.
Costa, A.S.
Nucci, M.
Alviano, C.S.
Rozental, S.
author_facet Ishida, K.
Alviano, D.S.
Silva, B.G.
Guerra, C.R.
Costa, A.S.
Nucci, M.
Alviano, C.S.
Rozental, S.
author_sort Ishida, K.
collection PubMed
description Fusarium species have emerged as one of the more outstanding groups of clinically important filamentous fungi, causing localized and life-threatening invasive infections with high morbidity and mortality. The ability to produce different types of hydrolytic enzymes is thought to be an important virulence mechanism of fungal pathogens and could be associated with the environment of the microorganism. Here, we have measured the production of two distinct lipolytic enzymes, phospholipase and esterase, by sixteen Fusarium isolates recovered from the hospital environment, immunocompromised patients' blood cultures, foot interdigital space scrapings from immunocompromised patients, and foot interdigital space scrapings from immunocompetent patients (4 isolates each). Fourteen of these 16 isolates were identified as Fusarium solani species complex (FSSC) and two were identified as F. oxysporum species complex (FOSC). Some relevant genus characteristics were visualized by light and electron microscopy such as curved and multicelled macroconidia with 3 or 4 septa, microconidia, phialides, and abundant chlamydospores. All Fusarium isolates were able to produce esterase and phospholipase under the experimental conditions. However, a negative correlation was observed between these two enzymes, indicating that a Fusarium isolate with high phospholipase activity has low esterase activity and vice versa. In addition, Fusarium isolated from clinical material produced more phospholipases, while environmental strains produced more esterases. These observations may be correlated with the different types of substrates that these fungi need to degrade during their nutrition processes.
format Online
Article
Text
id pubmed-3854292
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Sociedade Brasileira de Medicina Tropical
record_format MEDLINE/PubMed
spelling pubmed-38542922013-12-16 Negative correlation between phospholipase and esterase activity produced by Fusarium isolates Ishida, K. Alviano, D.S. Silva, B.G. Guerra, C.R. Costa, A.S. Nucci, M. Alviano, C.S. Rozental, S. Braz J Med Biol Res Short Communication Fusarium species have emerged as one of the more outstanding groups of clinically important filamentous fungi, causing localized and life-threatening invasive infections with high morbidity and mortality. The ability to produce different types of hydrolytic enzymes is thought to be an important virulence mechanism of fungal pathogens and could be associated with the environment of the microorganism. Here, we have measured the production of two distinct lipolytic enzymes, phospholipase and esterase, by sixteen Fusarium isolates recovered from the hospital environment, immunocompromised patients' blood cultures, foot interdigital space scrapings from immunocompromised patients, and foot interdigital space scrapings from immunocompetent patients (4 isolates each). Fourteen of these 16 isolates were identified as Fusarium solani species complex (FSSC) and two were identified as F. oxysporum species complex (FOSC). Some relevant genus characteristics were visualized by light and electron microscopy such as curved and multicelled macroconidia with 3 or 4 septa, microconidia, phialides, and abundant chlamydospores. All Fusarium isolates were able to produce esterase and phospholipase under the experimental conditions. However, a negative correlation was observed between these two enzymes, indicating that a Fusarium isolate with high phospholipase activity has low esterase activity and vice versa. In addition, Fusarium isolated from clinical material produced more phospholipases, while environmental strains produced more esterases. These observations may be correlated with the different types of substrates that these fungi need to degrade during their nutrition processes. Sociedade Brasileira de Medicina Tropical 2012-03-16 /pmc/articles/PMC3854292/ /pubmed/22415116 http://dx.doi.org/10.1590/S0100-879X2012007500034 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Ishida, K.
Alviano, D.S.
Silva, B.G.
Guerra, C.R.
Costa, A.S.
Nucci, M.
Alviano, C.S.
Rozental, S.
Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
title Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
title_full Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
title_fullStr Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
title_full_unstemmed Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
title_short Negative correlation between phospholipase and esterase activity produced by Fusarium isolates
title_sort negative correlation between phospholipase and esterase activity produced by fusarium isolates
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854292/
https://www.ncbi.nlm.nih.gov/pubmed/22415116
http://dx.doi.org/10.1590/S0100-879X2012007500034
work_keys_str_mv AT ishidak negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT alvianods negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT silvabg negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT guerracr negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT costaas negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT nuccim negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT alvianocs negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates
AT rozentals negativecorrelationbetweenphospholipaseandesteraseactivityproducedbyfusariumisolates