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Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium
This study explored the reduction of adenosine triphosphate (ATP) levels in L-02 hepatocytes by hexavalent chromium (Cr(VI)) using chi-square analysis. Cells were treated with 2, 4, 8, 16, or 32 µM Cr(VI) for 12, 24, or 36 h. Methyl thiazolyl tetrazolium (MTT) experiments and measurements of intrace...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Medicina Tropical
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854294/ https://www.ncbi.nlm.nih.gov/pubmed/22437481 http://dx.doi.org/10.1590/S0100-879X2012007500040 |
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author | Yang, Yuan Li, Peng Hu, Gong-Hua Dai, Lu Zhong, Xia-Li Zhou, Yu Zhong, Cai-Gao |
author_facet | Yang, Yuan Li, Peng Hu, Gong-Hua Dai, Lu Zhong, Xia-Li Zhou, Yu Zhong, Cai-Gao |
author_sort | Yang, Yuan |
collection | PubMed |
description | This study explored the reduction of adenosine triphosphate (ATP) levels in L-02 hepatocytes by hexavalent chromium (Cr(VI)) using chi-square analysis. Cells were treated with 2, 4, 8, 16, or 32 µM Cr(VI) for 12, 24, or 36 h. Methyl thiazolyl tetrazolium (MTT) experiments and measurements of intracellular ATP levels were performed by spectrophotometry or bioluminescence assays following Cr(VI) treatment. The chi-square test was used to determine the difference between cell survival rate and ATP levels. For the chi-square analysis, the results of the MTT or ATP experiments were transformed into a relative ratio with respect to the control (%). The relative ATP levels increased at 12 h, decreased at 24 h, and increased slightly again at 36 h following 4, 8, 16, 32 µM Cr(VI) treatment, corresponding to a “V-shaped” curve. Furthermore, the results of the chi-square analysis demonstrated a significant difference of the ATP level in the 32-µM Cr(VI) group (P < 0.05). The results suggest that the chi-square test can be applied to analyze the interference effects of Cr(VI) on ATP levels in L-02 hepatocytes. The decreased ATP levels at 24 h indicated disruption of mitochondrial energy metabolism and the slight increase of ATP levels at 36 h indicated partial recovery of mitochondrial function or activated glycolysis in L-02 hepatocytes. |
format | Online Article Text |
id | pubmed-3854294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-38542942013-12-16 Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium Yang, Yuan Li, Peng Hu, Gong-Hua Dai, Lu Zhong, Xia-Li Zhou, Yu Zhong, Cai-Gao Braz J Med Biol Res Short Communication This study explored the reduction of adenosine triphosphate (ATP) levels in L-02 hepatocytes by hexavalent chromium (Cr(VI)) using chi-square analysis. Cells were treated with 2, 4, 8, 16, or 32 µM Cr(VI) for 12, 24, or 36 h. Methyl thiazolyl tetrazolium (MTT) experiments and measurements of intracellular ATP levels were performed by spectrophotometry or bioluminescence assays following Cr(VI) treatment. The chi-square test was used to determine the difference between cell survival rate and ATP levels. For the chi-square analysis, the results of the MTT or ATP experiments were transformed into a relative ratio with respect to the control (%). The relative ATP levels increased at 12 h, decreased at 24 h, and increased slightly again at 36 h following 4, 8, 16, 32 µM Cr(VI) treatment, corresponding to a “V-shaped” curve. Furthermore, the results of the chi-square analysis demonstrated a significant difference of the ATP level in the 32-µM Cr(VI) group (P < 0.05). The results suggest that the chi-square test can be applied to analyze the interference effects of Cr(VI) on ATP levels in L-02 hepatocytes. The decreased ATP levels at 24 h indicated disruption of mitochondrial energy metabolism and the slight increase of ATP levels at 36 h indicated partial recovery of mitochondrial function or activated glycolysis in L-02 hepatocytes. Sociedade Brasileira de Medicina Tropical 2012-03-23 /pmc/articles/PMC3854294/ /pubmed/22437481 http://dx.doi.org/10.1590/S0100-879X2012007500040 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Yang, Yuan Li, Peng Hu, Gong-Hua Dai, Lu Zhong, Xia-Li Zhou, Yu Zhong, Cai-Gao Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium |
title | Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium |
title_full | Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium |
title_fullStr | Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium |
title_full_unstemmed | Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium |
title_short | Chi-square analysis of the reduction of ATP levels in L-02 hepatocytes by hexavalent chromium |
title_sort | chi-square analysis of the reduction of atp levels in l-02 hepatocytes by hexavalent chromium |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854294/ https://www.ncbi.nlm.nih.gov/pubmed/22437481 http://dx.doi.org/10.1590/S0100-879X2012007500040 |
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