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In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics

In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in v...

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Autores principales: Sun, Dao-Cai, Li, De-Hua, Ji, Hui-Cang, Rao, Guo-Zhou, Liang, Li-Hua, Ma, Ai-Jie, Xie, Chao, Zou, Gui-Ke, Song, Ying-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854304/
https://www.ncbi.nlm.nih.gov/pubmed/22473318
http://dx.doi.org/10.1590/S0100-879X2012007500054
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author Sun, Dao-Cai
Li, De-Hua
Ji, Hui-Cang
Rao, Guo-Zhou
Liang, Li-Hua
Ma, Ai-Jie
Xie, Chao
Zou, Gui-Ke
Song, Ying-Liang
author_facet Sun, Dao-Cai
Li, De-Hua
Ji, Hui-Cang
Rao, Guo-Zhou
Liang, Li-Hua
Ma, Ai-Jie
Xie, Chao
Zou, Gui-Ke
Song, Ying-Liang
author_sort Sun, Dao-Cai
collection PubMed
description In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant. Furthermore, we detected the adhesion ability of two types of cells from titanium slices using non-specific immunofluorescence staining and cell count. The two cell forms showed no significant difference in morphology under the same culture conditions. However, the alveolar bone osteoblasts received from type 2 diabetic patients had slower growth, lower cell activity and calcium nodule formation than the normal ones. The concentration of ALP, BGP and COL-I was lower in the supernatant of alveolar bone osteoblasts received from type 2 diabetic patients than in that received from normal subjects (P < 0.05). The alveolar bone osteoblasts obtained from type 2 diabetic patients can be successfully cultured in vitro with the same morphology and biological characteristics as those from normal patients, but with slower growth and lower concentration of specific secretion and lower combining ability with titanium than normal ones.
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spelling pubmed-38543042013-12-16 In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics Sun, Dao-Cai Li, De-Hua Ji, Hui-Cang Rao, Guo-Zhou Liang, Li-Hua Ma, Ai-Jie Xie, Chao Zou, Gui-Ke Song, Ying-Liang Braz J Med Biol Res Short Communication In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant. Furthermore, we detected the adhesion ability of two types of cells from titanium slices using non-specific immunofluorescence staining and cell count. The two cell forms showed no significant difference in morphology under the same culture conditions. However, the alveolar bone osteoblasts received from type 2 diabetic patients had slower growth, lower cell activity and calcium nodule formation than the normal ones. The concentration of ALP, BGP and COL-I was lower in the supernatant of alveolar bone osteoblasts received from type 2 diabetic patients than in that received from normal subjects (P < 0.05). The alveolar bone osteoblasts obtained from type 2 diabetic patients can be successfully cultured in vitro with the same morphology and biological characteristics as those from normal patients, but with slower growth and lower concentration of specific secretion and lower combining ability with titanium than normal ones. Sociedade Brasileira de Medicina Tropical 2012-04-05 /pmc/articles/PMC3854304/ /pubmed/22473318 http://dx.doi.org/10.1590/S0100-879X2012007500054 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Sun, Dao-Cai
Li, De-Hua
Ji, Hui-Cang
Rao, Guo-Zhou
Liang, Li-Hua
Ma, Ai-Jie
Xie, Chao
Zou, Gui-Ke
Song, Ying-Liang
In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
title In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
title_full In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
title_fullStr In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
title_full_unstemmed In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
title_short In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
title_sort in vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854304/
https://www.ncbi.nlm.nih.gov/pubmed/22473318
http://dx.doi.org/10.1590/S0100-879X2012007500054
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