Cargando…
Sleep pattern and learning in knockdown mice with reduced cholinergic neurotransmission
Impaired cholinergic neurotransmission can affect memory formation and influence sleep-wake cycles (SWC). In the present study, we describe the SWC in mice with a deficient vesicular acetylcholine transporter (VAChT) system, previously characterized as presenting reduced acetylcholine release and co...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854314/ https://www.ncbi.nlm.nih.gov/pubmed/24141612 http://dx.doi.org/10.1590/1414-431X20133102 |
_version_ | 1782294780158410752 |
---|---|
author | Queiroz, C.M. Tiba, P.A. Moreira, K.M. Guidine, P.A.M. Rezende, G.H.S. Moraes, M.F.D. Prado, M.A.M. Prado, V.F. Tufik, S. Mello, L.E. |
author_facet | Queiroz, C.M. Tiba, P.A. Moreira, K.M. Guidine, P.A.M. Rezende, G.H.S. Moraes, M.F.D. Prado, M.A.M. Prado, V.F. Tufik, S. Mello, L.E. |
author_sort | Queiroz, C.M. |
collection | PubMed |
description | Impaired cholinergic neurotransmission can affect memory formation and influence sleep-wake cycles (SWC). In the present study, we describe the SWC in mice with a deficient vesicular acetylcholine transporter (VAChT) system, previously characterized as presenting reduced acetylcholine release and cognitive and behavioral dysfunctions. Continuous, chronic ECoG and EMG recordings were used to evaluate the SWC pattern during light and dark phases in VAChT knockdown heterozygous (VAChT-KD(HET), n=7) and wild-type (WT, n=7) mice. SWC were evaluated for sleep efficiency, total amount and mean duration of slow-wave, intermediate and paradoxical sleep, as well as the number of awakenings from sleep. After recording SWC, contextual fear-conditioning tests were used as an acetylcholine-dependent learning paradigm. The results showed that sleep efficiency in VAChT-KD(HET) animals was similar to that of WT mice, but that the SWC was more fragmented. Fragmentation was characterized by an increase in the number of awakenings, mainly during intermediate sleep. VAChT-KD(HET) animals performed poorly in the contextual fear-conditioning paradigm (mean freezing time: 34.4±3.1 and 44.5±3.3 s for WT and VAChT-KD(HET) animals, respectively), which was followed by a 45% reduction in the number of paradoxical sleep episodes after the training session. Taken together, the results show that reduced cholinergic transmission led to sleep fragmentation and learning impairment. We discuss the results on the basis of cholinergic plasticity and its relevance to sleep homeostasis. We suggest that VAChT-KD(HET) mice could be a useful model to test cholinergic drugs used to treat sleep dysfunction in neurodegenerative disorders. |
format | Online Article Text |
id | pubmed-3854314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-38543142013-12-16 Sleep pattern and learning in knockdown mice with reduced cholinergic neurotransmission Queiroz, C.M. Tiba, P.A. Moreira, K.M. Guidine, P.A.M. Rezende, G.H.S. Moraes, M.F.D. Prado, M.A.M. Prado, V.F. Tufik, S. Mello, L.E. Braz J Med Biol Res Biomedical Sciences Impaired cholinergic neurotransmission can affect memory formation and influence sleep-wake cycles (SWC). In the present study, we describe the SWC in mice with a deficient vesicular acetylcholine transporter (VAChT) system, previously characterized as presenting reduced acetylcholine release and cognitive and behavioral dysfunctions. Continuous, chronic ECoG and EMG recordings were used to evaluate the SWC pattern during light and dark phases in VAChT knockdown heterozygous (VAChT-KD(HET), n=7) and wild-type (WT, n=7) mice. SWC were evaluated for sleep efficiency, total amount and mean duration of slow-wave, intermediate and paradoxical sleep, as well as the number of awakenings from sleep. After recording SWC, contextual fear-conditioning tests were used as an acetylcholine-dependent learning paradigm. The results showed that sleep efficiency in VAChT-KD(HET) animals was similar to that of WT mice, but that the SWC was more fragmented. Fragmentation was characterized by an increase in the number of awakenings, mainly during intermediate sleep. VAChT-KD(HET) animals performed poorly in the contextual fear-conditioning paradigm (mean freezing time: 34.4±3.1 and 44.5±3.3 s for WT and VAChT-KD(HET) animals, respectively), which was followed by a 45% reduction in the number of paradoxical sleep episodes after the training session. Taken together, the results show that reduced cholinergic transmission led to sleep fragmentation and learning impairment. We discuss the results on the basis of cholinergic plasticity and its relevance to sleep homeostasis. We suggest that VAChT-KD(HET) mice could be a useful model to test cholinergic drugs used to treat sleep dysfunction in neurodegenerative disorders. Associação Brasileira de Divulgação Científica 2013-10-02 /pmc/articles/PMC3854314/ /pubmed/24141612 http://dx.doi.org/10.1590/1414-431X20133102 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedical Sciences Queiroz, C.M. Tiba, P.A. Moreira, K.M. Guidine, P.A.M. Rezende, G.H.S. Moraes, M.F.D. Prado, M.A.M. Prado, V.F. Tufik, S. Mello, L.E. Sleep pattern and learning in knockdown mice with reduced cholinergic neurotransmission |
title | Sleep pattern and learning in knockdown mice with
reduced cholinergic neurotransmission |
title_full | Sleep pattern and learning in knockdown mice with
reduced cholinergic neurotransmission |
title_fullStr | Sleep pattern and learning in knockdown mice with
reduced cholinergic neurotransmission |
title_full_unstemmed | Sleep pattern and learning in knockdown mice with
reduced cholinergic neurotransmission |
title_short | Sleep pattern and learning in knockdown mice with
reduced cholinergic neurotransmission |
title_sort | sleep pattern and learning in knockdown mice with
reduced cholinergic neurotransmission |
topic | Biomedical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854314/ https://www.ncbi.nlm.nih.gov/pubmed/24141612 http://dx.doi.org/10.1590/1414-431X20133102 |
work_keys_str_mv | AT queirozcm sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT tibapa sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT moreirakm sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT guidinepam sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT rezendeghs sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT moraesmfd sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT pradomam sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT pradovf sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT tufiks sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission AT mellole sleeppatternandlearninginknockdownmicewithreducedcholinergicneurotransmission |