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Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells
Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Associação Brasileira de Divulgação Científica
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854438/ https://www.ncbi.nlm.nih.gov/pubmed/23780424 http://dx.doi.org/10.1590/1414-431X20131662 |
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author | Yin, Wanzhong Wang, Ping Wang, Xin Song, Wenzhi Cui, Xiangyan Yu, Hong Zhu, Wei |
author_facet | Yin, Wanzhong Wang, Ping Wang, Xin Song, Wenzhi Cui, Xiangyan Yu, Hong Zhu, Wei |
author_sort | Yin, Wanzhong |
collection | PubMed |
description | Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h, P<0.05), higher percentage of cells in G0/G1 phase (80.98±0.52 vs 69.14±0.89, P<0.05), increased efflux of rhodamine 123 (95.97±0.56 vs 12.40±0.44%, P<0.01), and up-regulated MDR1 expression. A total of 7 microRNAs and 605 mRNAs were differentially expressed between the two cell types. Of the differentially expressed mRNAs identified, regulator of G-protein signaling 10, high-temperature requirement protein A1, and nuclear protein 1 were found to be the putative targets of the differentially expressed microRNAs identified. These findings may open a new avenue for clarifying the mechanisms responsible for MDR in laryngeal cancer. |
format | Online Article Text |
id | pubmed-3854438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-38544382013-12-16 Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells Yin, Wanzhong Wang, Ping Wang, Xin Song, Wenzhi Cui, Xiangyan Yu, Hong Zhu, Wei Braz J Med Biol Res Clinical Investigation Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h, P<0.05), higher percentage of cells in G0/G1 phase (80.98±0.52 vs 69.14±0.89, P<0.05), increased efflux of rhodamine 123 (95.97±0.56 vs 12.40±0.44%, P<0.01), and up-regulated MDR1 expression. A total of 7 microRNAs and 605 mRNAs were differentially expressed between the two cell types. Of the differentially expressed mRNAs identified, regulator of G-protein signaling 10, high-temperature requirement protein A1, and nuclear protein 1 were found to be the putative targets of the differentially expressed microRNAs identified. These findings may open a new avenue for clarifying the mechanisms responsible for MDR in laryngeal cancer. Associação Brasileira de Divulgação Científica 2013-06-12 /pmc/articles/PMC3854438/ /pubmed/23780424 http://dx.doi.org/10.1590/1414-431X20131662 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Investigation Yin, Wanzhong Wang, Ping Wang, Xin Song, Wenzhi Cui, Xiangyan Yu, Hong Zhu, Wei Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells |
title | Identification of microRNAs and mRNAs associated with
multidrug resistance of human laryngeal cancer Hep-2 cells |
title_full | Identification of microRNAs and mRNAs associated with
multidrug resistance of human laryngeal cancer Hep-2 cells |
title_fullStr | Identification of microRNAs and mRNAs associated with
multidrug resistance of human laryngeal cancer Hep-2 cells |
title_full_unstemmed | Identification of microRNAs and mRNAs associated with
multidrug resistance of human laryngeal cancer Hep-2 cells |
title_short | Identification of microRNAs and mRNAs associated with
multidrug resistance of human laryngeal cancer Hep-2 cells |
title_sort | identification of micrornas and mrnas associated with
multidrug resistance of human laryngeal cancer hep-2 cells |
topic | Clinical Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854438/ https://www.ncbi.nlm.nih.gov/pubmed/23780424 http://dx.doi.org/10.1590/1414-431X20131662 |
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