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Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA
BACKGROUND: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tib...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854541/ https://www.ncbi.nlm.nih.gov/pubmed/24160332 http://dx.doi.org/10.1186/1752-153X-7-170 |
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author | Wang, Zhe Ma, Pei Xu, Lijia He, Chunnian Peng, Yong Xiao, Peigen |
author_facet | Wang, Zhe Ma, Pei Xu, Lijia He, Chunnian Peng, Yong Xiao, Peigen |
author_sort | Wang, Zhe |
collection | PubMed |
description | BACKGROUND: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone glycosides are a series of major active ingredients found in all three species. They are key intermediates in the anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic. RESULTS: A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six anthraquinone glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the anthraquinone glycoside content varied significantly even within the same species. The results showed that the anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher anthraquinone glycoside content. CONCLUSIONS: This work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six anthraquinone glycosides in rhubarb. The anthraquinone glycoside content varied significantly within the same species. The relationship of the anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six anthraquinone glycosides in rhubarb. The variations in the anthraquinone glycoside content were primarily due to the different altitude where the plant was grown. |
format | Online Article Text |
id | pubmed-3854541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38545412013-12-07 Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA Wang, Zhe Ma, Pei Xu, Lijia He, Chunnian Peng, Yong Xiao, Peigen Chem Cent J Research Article BACKGROUND: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone glycosides are a series of major active ingredients found in all three species. They are key intermediates in the anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic. RESULTS: A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six anthraquinone glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the anthraquinone glycoside content varied significantly even within the same species. The results showed that the anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher anthraquinone glycoside content. CONCLUSIONS: This work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six anthraquinone glycosides in rhubarb. The anthraquinone glycoside content varied significantly within the same species. The relationship of the anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six anthraquinone glycosides in rhubarb. The variations in the anthraquinone glycoside content were primarily due to the different altitude where the plant was grown. BioMed Central 2013-10-26 /pmc/articles/PMC3854541/ /pubmed/24160332 http://dx.doi.org/10.1186/1752-153X-7-170 Text en Copyright © 2013 Wang et al.; licensee Chemistry Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Zhe Ma, Pei Xu, Lijia He, Chunnian Peng, Yong Xiao, Peigen Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA |
title | Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA |
title_full | Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA |
title_fullStr | Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA |
title_full_unstemmed | Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA |
title_short | Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDA |
title_sort | evaluation of the content variation of anthraquinone glycosides in rhubarb by uplc-pda |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854541/ https://www.ncbi.nlm.nih.gov/pubmed/24160332 http://dx.doi.org/10.1186/1752-153X-7-170 |
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