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Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser
Corneal grafts for Descemet’s Stripping Automated Endothelial Keratoplasty are commonly prepared using mechanical microkeratomes. However, the cuts produced in such way render corneal lenticules that are thinner centrally than peripherally, thus inducing a hyperopic shift. Here we describe a novel d...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855481/ https://www.ncbi.nlm.nih.gov/pubmed/24340090 http://dx.doi.org/10.1371/journal.pone.0083185 |
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author | Singh, Kanwarpal Haydari, Nour Brunette, Isabelle Costantino, Santiago |
author_facet | Singh, Kanwarpal Haydari, Nour Brunette, Isabelle Costantino, Santiago |
author_sort | Singh, Kanwarpal |
collection | PubMed |
description | Corneal grafts for Descemet’s Stripping Automated Endothelial Keratoplasty are commonly prepared using mechanical microkeratomes. However, the cuts produced in such way render corneal lenticules that are thinner centrally than peripherally, thus inducing a hyperopic shift. Here we describe a novel device for preparing donor corneal grafts, in which a single low-energy femtosecond laser system is used as both a light source for optical coherence tomography and for cutting the graft illuminating from the endothelial side. The same laser is first utilized to obtain three-dimensional optical coherence tomography images of the donor tissue for guiding the dissection and obtaining grafts of uniform thickness with no applanation or contact. This device allows an optimal procedure for preparing consistently thin posterior grafts for transplantation. |
format | Online Article Text |
id | pubmed-3855481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38554812013-12-11 Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser Singh, Kanwarpal Haydari, Nour Brunette, Isabelle Costantino, Santiago PLoS One Research Article Corneal grafts for Descemet’s Stripping Automated Endothelial Keratoplasty are commonly prepared using mechanical microkeratomes. However, the cuts produced in such way render corneal lenticules that are thinner centrally than peripherally, thus inducing a hyperopic shift. Here we describe a novel device for preparing donor corneal grafts, in which a single low-energy femtosecond laser system is used as both a light source for optical coherence tomography and for cutting the graft illuminating from the endothelial side. The same laser is first utilized to obtain three-dimensional optical coherence tomography images of the donor tissue for guiding the dissection and obtaining grafts of uniform thickness with no applanation or contact. This device allows an optimal procedure for preparing consistently thin posterior grafts for transplantation. Public Library of Science 2013-12-05 /pmc/articles/PMC3855481/ /pubmed/24340090 http://dx.doi.org/10.1371/journal.pone.0083185 Text en © 2013 Singh et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Singh, Kanwarpal Haydari, Nour Brunette, Isabelle Costantino, Santiago Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser |
title | Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser |
title_full | Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser |
title_fullStr | Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser |
title_full_unstemmed | Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser |
title_short | Preparing Uniform-Thickness Corneal Endothelial Grafts from Donor Tissues Using a Non-Amplified Femtosecond Laser |
title_sort | preparing uniform-thickness corneal endothelial grafts from donor tissues using a non-amplified femtosecond laser |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855481/ https://www.ncbi.nlm.nih.gov/pubmed/24340090 http://dx.doi.org/10.1371/journal.pone.0083185 |
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