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Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c
Racial differences in the pathophysiology of atherothrombosis are poorly understood. We explored the function and transcriptome of platelets in healthy black (n = 70) and white (n = 84) subjects. PAR4 thrombin receptor induced platelet aggregation and calcium mobilization were significantly greater...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855898/ https://www.ncbi.nlm.nih.gov/pubmed/24216752 http://dx.doi.org/10.1038/nm.3385 |
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author | Edelstein, Leonard C. Simon, Lukas M. Montoya, Raúl Teruel Holinstat, Michael Chen, Edward S. Bergeron, Angela Kong, Xianguo Nagalla, Srikanth Mohandas, Narla Cohen, David E. Dong, Jing-fei Shaw, Chad Bray, Paul F. |
author_facet | Edelstein, Leonard C. Simon, Lukas M. Montoya, Raúl Teruel Holinstat, Michael Chen, Edward S. Bergeron, Angela Kong, Xianguo Nagalla, Srikanth Mohandas, Narla Cohen, David E. Dong, Jing-fei Shaw, Chad Bray, Paul F. |
author_sort | Edelstein, Leonard C. |
collection | PubMed |
description | Racial differences in the pathophysiology of atherothrombosis are poorly understood. We explored the function and transcriptome of platelets in healthy black (n = 70) and white (n = 84) subjects. PAR4 thrombin receptor induced platelet aggregation and calcium mobilization were significantly greater in black subjects. Numerous differentially expressed (DE) RNAs were associated with both race and PAR4 reactivity, including phosphatidylcholine transfer protein (PCTP), and platelets from blacks expressed higher levels of PC-TP protein. PC-TP inhibition or depletion blocked activation of platelets or megakaryocytic cell lines through PAR4 but not PAR1. MiR-376c levels were DE by race and PAR4 reactivity, and were inversely correlated with PCTP mRNA levels, PC-TP protein levels and PAR4 reactivity. MiR-376c regulated expression of PC-TP in human megakaryocytes. A disproportionately high number of miRNAs DE by race and PAR4 reactivity, including miR-376c, are encoded in the DLK1-DIO3 locus, and were lower in platelets from blacks. These results support PC-TP as a regulator of the racial difference in PAR4-mediated platelet activation, indicate a genomic contribution to platelet function that differs by race, and emphasize a need to consider race effects when developing anti-thrombotic drugs. |
format | Online Article Text |
id | pubmed-3855898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-38558982014-06-01 Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c Edelstein, Leonard C. Simon, Lukas M. Montoya, Raúl Teruel Holinstat, Michael Chen, Edward S. Bergeron, Angela Kong, Xianguo Nagalla, Srikanth Mohandas, Narla Cohen, David E. Dong, Jing-fei Shaw, Chad Bray, Paul F. Nat Med Article Racial differences in the pathophysiology of atherothrombosis are poorly understood. We explored the function and transcriptome of platelets in healthy black (n = 70) and white (n = 84) subjects. PAR4 thrombin receptor induced platelet aggregation and calcium mobilization were significantly greater in black subjects. Numerous differentially expressed (DE) RNAs were associated with both race and PAR4 reactivity, including phosphatidylcholine transfer protein (PCTP), and platelets from blacks expressed higher levels of PC-TP protein. PC-TP inhibition or depletion blocked activation of platelets or megakaryocytic cell lines through PAR4 but not PAR1. MiR-376c levels were DE by race and PAR4 reactivity, and were inversely correlated with PCTP mRNA levels, PC-TP protein levels and PAR4 reactivity. MiR-376c regulated expression of PC-TP in human megakaryocytes. A disproportionately high number of miRNAs DE by race and PAR4 reactivity, including miR-376c, are encoded in the DLK1-DIO3 locus, and were lower in platelets from blacks. These results support PC-TP as a regulator of the racial difference in PAR4-mediated platelet activation, indicate a genomic contribution to platelet function that differs by race, and emphasize a need to consider race effects when developing anti-thrombotic drugs. 2013-11-10 2013-12 /pmc/articles/PMC3855898/ /pubmed/24216752 http://dx.doi.org/10.1038/nm.3385 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Edelstein, Leonard C. Simon, Lukas M. Montoya, Raúl Teruel Holinstat, Michael Chen, Edward S. Bergeron, Angela Kong, Xianguo Nagalla, Srikanth Mohandas, Narla Cohen, David E. Dong, Jing-fei Shaw, Chad Bray, Paul F. Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c |
title | Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c |
title_full | Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c |
title_fullStr | Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c |
title_full_unstemmed | Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c |
title_short | Racial Difference in Human Platelet PAR4 Reactivity Reflects Expression of PCTP and miR-376c |
title_sort | racial difference in human platelet par4 reactivity reflects expression of pctp and mir-376c |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855898/ https://www.ncbi.nlm.nih.gov/pubmed/24216752 http://dx.doi.org/10.1038/nm.3385 |
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