Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation

Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using th...

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Autores principales: Wang, Yan, Xu, Chang, Du, Li Qing, Cao, Jia, Liu, Jian Xiang, Su, Xu, Zhao, Hui, Fan, Fei-Yue, Wang, Bing, Katsube, Takanori, Fan, Sai Jun, Liu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856073/
https://www.ncbi.nlm.nih.gov/pubmed/24240807
http://dx.doi.org/10.3390/ijms141122449
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author Wang, Yan
Xu, Chang
Du, Li Qing
Cao, Jia
Liu, Jian Xiang
Su, Xu
Zhao, Hui
Fan, Fei-Yue
Wang, Bing
Katsube, Takanori
Fan, Sai Jun
Liu, Qiang
author_facet Wang, Yan
Xu, Chang
Du, Li Qing
Cao, Jia
Liu, Jian Xiang
Su, Xu
Zhao, Hui
Fan, Fei-Yue
Wang, Bing
Katsube, Takanori
Fan, Sai Jun
Liu, Qiang
author_sort Wang, Yan
collection PubMed
description Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
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spelling pubmed-38560732013-12-09 Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation Wang, Yan Xu, Chang Du, Li Qing Cao, Jia Liu, Jian Xiang Su, Xu Zhao, Hui Fan, Fei-Yue Wang, Bing Katsube, Takanori Fan, Sai Jun Liu, Qiang Int J Mol Sci Article Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined. Molecular Diversity Preservation International (MDPI) 2013-11-14 /pmc/articles/PMC3856073/ /pubmed/24240807 http://dx.doi.org/10.3390/ijms141122449 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Yan
Xu, Chang
Du, Li Qing
Cao, Jia
Liu, Jian Xiang
Su, Xu
Zhao, Hui
Fan, Fei-Yue
Wang, Bing
Katsube, Takanori
Fan, Sai Jun
Liu, Qiang
Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
title Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
title_full Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
title_fullStr Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
title_full_unstemmed Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
title_short Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
title_sort evaluation of the comet assay for assessing the dose-response relationship of dna damage induced by ionizing radiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856073/
https://www.ncbi.nlm.nih.gov/pubmed/24240807
http://dx.doi.org/10.3390/ijms141122449
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