Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization

Infrared (IR) spectromicroscopy, or chemical imaging, is an evolving technique that is poised to make significant contributions in the fields of biology and medicine. Recent developments in sources, detectors, measurement techniques and speciman holders have now made diffraction-limited Fourier tran...

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Detalles Bibliográficos
Autores principales: Mattson, Eric C., Aboualizadeh, Ebrahim, Barabas, Marie E., Stucky, Cheryl L., Hirschmugl, Carol J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856089/
https://www.ncbi.nlm.nih.gov/pubmed/24256815
http://dx.doi.org/10.3390/ijms141122753
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author Mattson, Eric C.
Aboualizadeh, Ebrahim
Barabas, Marie E.
Stucky, Cheryl L.
Hirschmugl, Carol J.
author_facet Mattson, Eric C.
Aboualizadeh, Ebrahim
Barabas, Marie E.
Stucky, Cheryl L.
Hirschmugl, Carol J.
author_sort Mattson, Eric C.
collection PubMed
description Infrared (IR) spectromicroscopy, or chemical imaging, is an evolving technique that is poised to make significant contributions in the fields of biology and medicine. Recent developments in sources, detectors, measurement techniques and speciman holders have now made diffraction-limited Fourier transform infrared (FTIR) imaging of cellular chemistry in living cells a reality. The availability of bright, broadband IR sources and large area, pixelated detectors facilitate live cell imaging, which requires rapid measurements using non-destructive probes. In this work, we review advances in the field of FTIR spectromicroscopy that have contributed to live-cell two and three-dimensional IR imaging, and discuss several key examples that highlight the utility of this technique for studying the structure and chemistry of living cells.
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spelling pubmed-38560892013-12-09 Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization Mattson, Eric C. Aboualizadeh, Ebrahim Barabas, Marie E. Stucky, Cheryl L. Hirschmugl, Carol J. Int J Mol Sci Review Infrared (IR) spectromicroscopy, or chemical imaging, is an evolving technique that is poised to make significant contributions in the fields of biology and medicine. Recent developments in sources, detectors, measurement techniques and speciman holders have now made diffraction-limited Fourier transform infrared (FTIR) imaging of cellular chemistry in living cells a reality. The availability of bright, broadband IR sources and large area, pixelated detectors facilitate live cell imaging, which requires rapid measurements using non-destructive probes. In this work, we review advances in the field of FTIR spectromicroscopy that have contributed to live-cell two and three-dimensional IR imaging, and discuss several key examples that highlight the utility of this technique for studying the structure and chemistry of living cells. Molecular Diversity Preservation International (MDPI) 2013-11-19 /pmc/articles/PMC3856089/ /pubmed/24256815 http://dx.doi.org/10.3390/ijms141122753 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Mattson, Eric C.
Aboualizadeh, Ebrahim
Barabas, Marie E.
Stucky, Cheryl L.
Hirschmugl, Carol J.
Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
title Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
title_full Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
title_fullStr Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
title_full_unstemmed Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
title_short Opportunities for Live Cell FT-Infrared Imaging: Macromolecule Identification with 2D and 3D Localization
title_sort opportunities for live cell ft-infrared imaging: macromolecule identification with 2d and 3d localization
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856089/
https://www.ncbi.nlm.nih.gov/pubmed/24256815
http://dx.doi.org/10.3390/ijms141122753
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