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New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein
Polypyrimidine Tract Binding Protein (PTB) is an intensely studied RNA binding protein involved in several post-transcriptional regulatory events of gene expression. Initially described as a pre-mRNA splicing regulator, PTB is now widely accepted as a multifunctional protein shuttling between nucleu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856098/ https://www.ncbi.nlm.nih.gov/pubmed/24264039 http://dx.doi.org/10.3390/ijms141122906 |
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author | Romanelli, Maria Grazia Diani, Erica Lievens, Patricia Marie-Jeanne |
author_facet | Romanelli, Maria Grazia Diani, Erica Lievens, Patricia Marie-Jeanne |
author_sort | Romanelli, Maria Grazia |
collection | PubMed |
description | Polypyrimidine Tract Binding Protein (PTB) is an intensely studied RNA binding protein involved in several post-transcriptional regulatory events of gene expression. Initially described as a pre-mRNA splicing regulator, PTB is now widely accepted as a multifunctional protein shuttling between nucleus and cytoplasm. Accordingly, PTB can interact with selected RNA targets, structural elements and proteins. There is increasing evidence that PTB and its paralog PTBP2 play a major role as repressors of alternatively spliced exons, whose transcription is tissue-regulated. In addition to alternative splicing, PTB is involved in almost all steps of mRNA metabolism, including polyadenylation, mRNA stability and initiation of protein translation. Furthermore, it is well established that PTB recruitment in internal ribosome entry site (IRES) activates the translation of picornaviral and cellular proteins. Detailed studies of the structural properties of PTB have contributed to our understanding of the mechanism of RNA binding by RNA Recognition Motif (RRM) domains. In the present review, we will describe the structural properties of PTB, its paralogs and co-factors, the role in post-transcriptional regulation and actions in cell differentiation and pathogenesis. Defining the multifunctional roles of PTB will contribute to the understanding of key regulatory events in gene expression. |
format | Online Article Text |
id | pubmed-3856098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-38560982013-12-09 New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein Romanelli, Maria Grazia Diani, Erica Lievens, Patricia Marie-Jeanne Int J Mol Sci Review Polypyrimidine Tract Binding Protein (PTB) is an intensely studied RNA binding protein involved in several post-transcriptional regulatory events of gene expression. Initially described as a pre-mRNA splicing regulator, PTB is now widely accepted as a multifunctional protein shuttling between nucleus and cytoplasm. Accordingly, PTB can interact with selected RNA targets, structural elements and proteins. There is increasing evidence that PTB and its paralog PTBP2 play a major role as repressors of alternatively spliced exons, whose transcription is tissue-regulated. In addition to alternative splicing, PTB is involved in almost all steps of mRNA metabolism, including polyadenylation, mRNA stability and initiation of protein translation. Furthermore, it is well established that PTB recruitment in internal ribosome entry site (IRES) activates the translation of picornaviral and cellular proteins. Detailed studies of the structural properties of PTB have contributed to our understanding of the mechanism of RNA binding by RNA Recognition Motif (RRM) domains. In the present review, we will describe the structural properties of PTB, its paralogs and co-factors, the role in post-transcriptional regulation and actions in cell differentiation and pathogenesis. Defining the multifunctional roles of PTB will contribute to the understanding of key regulatory events in gene expression. Molecular Diversity Preservation International (MDPI) 2013-11-20 /pmc/articles/PMC3856098/ /pubmed/24264039 http://dx.doi.org/10.3390/ijms141122906 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Romanelli, Maria Grazia Diani, Erica Lievens, Patricia Marie-Jeanne New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein |
title | New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein |
title_full | New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein |
title_fullStr | New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein |
title_full_unstemmed | New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein |
title_short | New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein |
title_sort | new insights into functional roles of the polypyrimidine tract-binding protein |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856098/ https://www.ncbi.nlm.nih.gov/pubmed/24264039 http://dx.doi.org/10.3390/ijms141122906 |
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