Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification

We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 an...

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Autores principales: Yamamoto, Shoichiro, Kurokawa, Tadahide, Sekino, Masashi, Yasuike, Motoshige, Saitoh, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856111/
https://www.ncbi.nlm.nih.gov/pubmed/24284404
http://dx.doi.org/10.3390/ijms141123153
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author Yamamoto, Shoichiro
Kurokawa, Tadahide
Sekino, Masashi
Yasuike, Motoshige
Saitoh, Kenji
author_facet Yamamoto, Shoichiro
Kurokawa, Tadahide
Sekino, Masashi
Yasuike, Motoshige
Saitoh, Kenji
author_sort Yamamoto, Shoichiro
collection PubMed
description We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex.
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spelling pubmed-38561112013-12-09 Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification Yamamoto, Shoichiro Kurokawa, Tadahide Sekino, Masashi Yasuike, Motoshige Saitoh, Kenji Int J Mol Sci Article We developed tetranucleotide-repeat microsatellite markers for the masu salmon (Oncorhynchus masou) complex. 454 pyrosequencing was used to discover repeat motifs, and seven polymorphic microsatellite-primer sets were identified. The number of alleles detected at each locus ranged from four to 24 and the expected heterozygosity varied from 0.57 to 0.92. Cross-subspecies amplification for O. m. masou, O. m. ishikawae and O. m. subsp. was successful. These microsatellites can be utilized in studies of genetic structure, genetic diversity, and intra- and inter-subspecific hybridization, making a contribution to conservation and management of the Oncorhynchus masou complex. Molecular Diversity Preservation International (MDPI) 2013-11-21 /pmc/articles/PMC3856111/ /pubmed/24284404 http://dx.doi.org/10.3390/ijms141123153 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yamamoto, Shoichiro
Kurokawa, Tadahide
Sekino, Masashi
Yasuike, Motoshige
Saitoh, Kenji
Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
title Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
title_full Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
title_fullStr Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
title_full_unstemmed Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
title_short Tetra-Repeat Microsatellite Markers for the Masu Salmon (Oncorhynchus masou masou) and Its Application in Cross-Subspecies Amplification
title_sort tetra-repeat microsatellite markers for the masu salmon (oncorhynchus masou masou) and its application in cross-subspecies amplification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856111/
https://www.ncbi.nlm.nih.gov/pubmed/24284404
http://dx.doi.org/10.3390/ijms141123153
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