Cargando…
Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target
Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856372/ https://www.ncbi.nlm.nih.gov/pubmed/24324358 http://dx.doi.org/10.7150/ijms.6902 |
_version_ | 1782295047763394560 |
---|---|
author | Tan, Wee Chee Jaganath, Indu Bala Manikam, Rishya Sekaran, Shamala Devi |
author_facet | Tan, Wee Chee Jaganath, Indu Bala Manikam, Rishya Sekaran, Shamala Devi |
author_sort | Tan, Wee Chee |
collection | PubMed |
description | Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug and plants could be the potential lead. In this study, the antiviral activity of the aqueous extract of four Phyllanthus species were evaluated against herpes simplex virus type-1 (HSV-1) and HSV-2 in Vero cells by quantitative PCR. The protein expressions of untreated and treated infected Vero cells were studied by 2D-gel electrophoresis and Western blot. This is the first study that reported the antiviral activity of P. watsonii. P. urinaria was shown to demonstrate the strongest antiviral activity against HSV-1 and HSV-2, with SI >33.6. Time-of-addition studies suggested that the extract may act against the early infection stage and the replication stage. Protein expression studies indicated that cellular proteins that are involved in maintaining cytoskeletal structure could be potential target for development of antiviral drugs. Preliminary findings indicated that P. urinaria demonstrated potent inhibitory activity against HSV. Hence, further studies such as in vivo evaluation are required for the development of effective antiherpetic drug. |
format | Online Article Text |
id | pubmed-3856372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-38563722013-12-09 Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target Tan, Wee Chee Jaganath, Indu Bala Manikam, Rishya Sekaran, Shamala Devi Int J Med Sci Research Paper Nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant HSV strains particularly in immunocompromised patients, there is a need to develop an alternative antiherpetic drug and plants could be the potential lead. In this study, the antiviral activity of the aqueous extract of four Phyllanthus species were evaluated against herpes simplex virus type-1 (HSV-1) and HSV-2 in Vero cells by quantitative PCR. The protein expressions of untreated and treated infected Vero cells were studied by 2D-gel electrophoresis and Western blot. This is the first study that reported the antiviral activity of P. watsonii. P. urinaria was shown to demonstrate the strongest antiviral activity against HSV-1 and HSV-2, with SI >33.6. Time-of-addition studies suggested that the extract may act against the early infection stage and the replication stage. Protein expression studies indicated that cellular proteins that are involved in maintaining cytoskeletal structure could be potential target for development of antiviral drugs. Preliminary findings indicated that P. urinaria demonstrated potent inhibitory activity against HSV. Hence, further studies such as in vivo evaluation are required for the development of effective antiherpetic drug. Ivyspring International Publisher 2013-10-31 /pmc/articles/PMC3856372/ /pubmed/24324358 http://dx.doi.org/10.7150/ijms.6902 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Tan, Wee Chee Jaganath, Indu Bala Manikam, Rishya Sekaran, Shamala Devi Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target |
title | Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target |
title_full | Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target |
title_fullStr | Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target |
title_full_unstemmed | Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target |
title_short | Evaluation of Antiviral Activities of Four Local Malaysian Phyllanthus Species against Herpes Simplex Viruses and Possible Antiviral Target |
title_sort | evaluation of antiviral activities of four local malaysian phyllanthus species against herpes simplex viruses and possible antiviral target |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856372/ https://www.ncbi.nlm.nih.gov/pubmed/24324358 http://dx.doi.org/10.7150/ijms.6902 |
work_keys_str_mv | AT tanweechee evaluationofantiviralactivitiesoffourlocalmalaysianphyllanthusspeciesagainstherpessimplexvirusesandpossibleantiviraltarget AT jaganathindubala evaluationofantiviralactivitiesoffourlocalmalaysianphyllanthusspeciesagainstherpessimplexvirusesandpossibleantiviraltarget AT manikamrishya evaluationofantiviralactivitiesoffourlocalmalaysianphyllanthusspeciesagainstherpessimplexvirusesandpossibleantiviraltarget AT sekaranshamaladevi evaluationofantiviralactivitiesoffourlocalmalaysianphyllanthusspeciesagainstherpessimplexvirusesandpossibleantiviraltarget |