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Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells
Celecoxib, a cyclooxygenase-2 (COX-2) inhibitor, can elicit anti-tumor effects in various malignancies. Here, we sought to clarify the role of autophagy in celecoxib-induced cytotoxicity in human urothelial carcinoma (UC) cells. The results shows celecoxib induced cellular stress response such as en...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857231/ https://www.ncbi.nlm.nih.gov/pubmed/24349176 http://dx.doi.org/10.1371/journal.pone.0082034 |
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author | Huang, Kuo-How Kuo, Kuan-Lin Ho, I-Lin Chang, Hong-Chiang Chuang, Yuan-Ting Lin, Wei-Chou Lee, Ping-Yi Chang, Shih-Chen Chiang, Chih-Kang Pu, Yeong-Shiau Chou, Chien-Tso Hsu, Chen-Hsun Liu, Shing-Hwa |
author_facet | Huang, Kuo-How Kuo, Kuan-Lin Ho, I-Lin Chang, Hong-Chiang Chuang, Yuan-Ting Lin, Wei-Chou Lee, Ping-Yi Chang, Shih-Chen Chiang, Chih-Kang Pu, Yeong-Shiau Chou, Chien-Tso Hsu, Chen-Hsun Liu, Shing-Hwa |
author_sort | Huang, Kuo-How |
collection | PubMed |
description | Celecoxib, a cyclooxygenase-2 (COX-2) inhibitor, can elicit anti-tumor effects in various malignancies. Here, we sought to clarify the role of autophagy in celecoxib-induced cytotoxicity in human urothelial carcinoma (UC) cells. The results shows celecoxib induced cellular stress response such as endoplasmic reticulum (ER) stress, phosopho-SAPK/JNK, and phosopho-c-Jun as well as autophagosome formation in UC cells. Inhibition of autophagy by 3-methyladenine (3-MA), bafilomycin A1 or ATG7 knockdown potentiated celecoxib-induced apoptosis. Up-regulation of autophagy by rapamycin or GFP-LC3B-transfection alleviated celecoxib-induced cytotoxicity in UC cells. Taken together, the inhibition of autophagy enhances therapeutic efficacy of celecoxib in UC cells, suggesting a novel therapeutic strategy against UC. |
format | Online Article Text |
id | pubmed-3857231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38572312013-12-13 Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells Huang, Kuo-How Kuo, Kuan-Lin Ho, I-Lin Chang, Hong-Chiang Chuang, Yuan-Ting Lin, Wei-Chou Lee, Ping-Yi Chang, Shih-Chen Chiang, Chih-Kang Pu, Yeong-Shiau Chou, Chien-Tso Hsu, Chen-Hsun Liu, Shing-Hwa PLoS One Research Article Celecoxib, a cyclooxygenase-2 (COX-2) inhibitor, can elicit anti-tumor effects in various malignancies. Here, we sought to clarify the role of autophagy in celecoxib-induced cytotoxicity in human urothelial carcinoma (UC) cells. The results shows celecoxib induced cellular stress response such as endoplasmic reticulum (ER) stress, phosopho-SAPK/JNK, and phosopho-c-Jun as well as autophagosome formation in UC cells. Inhibition of autophagy by 3-methyladenine (3-MA), bafilomycin A1 or ATG7 knockdown potentiated celecoxib-induced apoptosis. Up-regulation of autophagy by rapamycin or GFP-LC3B-transfection alleviated celecoxib-induced cytotoxicity in UC cells. Taken together, the inhibition of autophagy enhances therapeutic efficacy of celecoxib in UC cells, suggesting a novel therapeutic strategy against UC. Public Library of Science 2013-12-09 /pmc/articles/PMC3857231/ /pubmed/24349176 http://dx.doi.org/10.1371/journal.pone.0082034 Text en © 2013 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Kuo-How Kuo, Kuan-Lin Ho, I-Lin Chang, Hong-Chiang Chuang, Yuan-Ting Lin, Wei-Chou Lee, Ping-Yi Chang, Shih-Chen Chiang, Chih-Kang Pu, Yeong-Shiau Chou, Chien-Tso Hsu, Chen-Hsun Liu, Shing-Hwa Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells |
title | Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells |
title_full | Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells |
title_fullStr | Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells |
title_full_unstemmed | Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells |
title_short | Celecoxib-Induced Cytotoxic Effect Is Potentiated by Inhibition of Autophagy in Human Urothelial Carcinoma Cells |
title_sort | celecoxib-induced cytotoxic effect is potentiated by inhibition of autophagy in human urothelial carcinoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857231/ https://www.ncbi.nlm.nih.gov/pubmed/24349176 http://dx.doi.org/10.1371/journal.pone.0082034 |
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