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Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
BACKGROUND: Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats. METHODS: Corneal transplants were perfo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857254/ https://www.ncbi.nlm.nih.gov/pubmed/24349336 http://dx.doi.org/10.1371/journal.pone.0082687 |
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author | Wacker, Katrin Denker, Sophy Hildebrand, Antonia Eberwein, Philipp Reinhard, Thomas Schwartzkopff, Johannes |
author_facet | Wacker, Katrin Denker, Sophy Hildebrand, Antonia Eberwein, Philipp Reinhard, Thomas Schwartzkopff, Johannes |
author_sort | Wacker, Katrin |
collection | PubMed |
description | BACKGROUND: Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats. METHODS: Corneal transplants were performed between Fisher-donor and Lewis-recipient rats. Recipients were postoperatively treated three times daily with AZM, miglyol, ofloxacin or dexamethasone eye drops. As an additional control, AZM was applied following syngeneic keratoplasty. Furthermore, short-term treatments with AZM for seven days perioperatively or with AZM only three days prior to the transplantation were compared to appropriate controls. All transplants were monitored clinically for opacity, edema, and vascularization. Infiltrating CD45(+), CD4(+), CD8(+), CD25(+), CD161(+) and CD163(+) cells were quantified via immunohistochemistry. RESULTS: AZM significantly promoted corneal graft survival compared with miglyol or ofloxacin treatment. This effect was comparable to topical dexamethasone. No adverse AZM effect was observed. Histology confirmed a significant reduction of infiltrating leukocytes. The short-term application of AZM for three days prior to transplantation or for seven days perioperatively reduced corneal graft rejection significantly compared with the controls. CONCLUSIONS: Along with antibiotic properties, topical AZM has a strong anti-inflammatory effect. Following keratoplasty, this effect is comparable to topical dexamethasone without the risk of steroid-induced adverse effects. Short-term treatment with AZM three days prior to the transplantation was sufficient to promote graft survival in the rat keratoplasty model. We therefore suggest further assessing the anti-inflammatory function of topical AZM following keratoplasty in humans. |
format | Online Article Text |
id | pubmed-3857254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38572542013-12-13 Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat Wacker, Katrin Denker, Sophy Hildebrand, Antonia Eberwein, Philipp Reinhard, Thomas Schwartzkopff, Johannes PLoS One Research Article BACKGROUND: Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats. METHODS: Corneal transplants were performed between Fisher-donor and Lewis-recipient rats. Recipients were postoperatively treated three times daily with AZM, miglyol, ofloxacin or dexamethasone eye drops. As an additional control, AZM was applied following syngeneic keratoplasty. Furthermore, short-term treatments with AZM for seven days perioperatively or with AZM only three days prior to the transplantation were compared to appropriate controls. All transplants were monitored clinically for opacity, edema, and vascularization. Infiltrating CD45(+), CD4(+), CD8(+), CD25(+), CD161(+) and CD163(+) cells were quantified via immunohistochemistry. RESULTS: AZM significantly promoted corneal graft survival compared with miglyol or ofloxacin treatment. This effect was comparable to topical dexamethasone. No adverse AZM effect was observed. Histology confirmed a significant reduction of infiltrating leukocytes. The short-term application of AZM for three days prior to transplantation or for seven days perioperatively reduced corneal graft rejection significantly compared with the controls. CONCLUSIONS: Along with antibiotic properties, topical AZM has a strong anti-inflammatory effect. Following keratoplasty, this effect is comparable to topical dexamethasone without the risk of steroid-induced adverse effects. Short-term treatment with AZM three days prior to the transplantation was sufficient to promote graft survival in the rat keratoplasty model. We therefore suggest further assessing the anti-inflammatory function of topical AZM following keratoplasty in humans. Public Library of Science 2013-12-09 /pmc/articles/PMC3857254/ /pubmed/24349336 http://dx.doi.org/10.1371/journal.pone.0082687 Text en © 2013 Wacker et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wacker, Katrin Denker, Sophy Hildebrand, Antonia Eberwein, Philipp Reinhard, Thomas Schwartzkopff, Johannes Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat |
title | Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat |
title_full | Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat |
title_fullStr | Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat |
title_full_unstemmed | Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat |
title_short | Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat |
title_sort | short-term azithromycin treatment promotes cornea allograft survival in the rat |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857254/ https://www.ncbi.nlm.nih.gov/pubmed/24349336 http://dx.doi.org/10.1371/journal.pone.0082687 |
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