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Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat

BACKGROUND: Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats. METHODS: Corneal transplants were perfo...

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Autores principales: Wacker, Katrin, Denker, Sophy, Hildebrand, Antonia, Eberwein, Philipp, Reinhard, Thomas, Schwartzkopff, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857254/
https://www.ncbi.nlm.nih.gov/pubmed/24349336
http://dx.doi.org/10.1371/journal.pone.0082687
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author Wacker, Katrin
Denker, Sophy
Hildebrand, Antonia
Eberwein, Philipp
Reinhard, Thomas
Schwartzkopff, Johannes
author_facet Wacker, Katrin
Denker, Sophy
Hildebrand, Antonia
Eberwein, Philipp
Reinhard, Thomas
Schwartzkopff, Johannes
author_sort Wacker, Katrin
collection PubMed
description BACKGROUND: Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats. METHODS: Corneal transplants were performed between Fisher-donor and Lewis-recipient rats. Recipients were postoperatively treated three times daily with AZM, miglyol, ofloxacin or dexamethasone eye drops. As an additional control, AZM was applied following syngeneic keratoplasty. Furthermore, short-term treatments with AZM for seven days perioperatively or with AZM only three days prior to the transplantation were compared to appropriate controls. All transplants were monitored clinically for opacity, edema, and vascularization. Infiltrating CD45(+), CD4(+), CD8(+), CD25(+), CD161(+) and CD163(+) cells were quantified via immunohistochemistry. RESULTS: AZM significantly promoted corneal graft survival compared with miglyol or ofloxacin treatment. This effect was comparable to topical dexamethasone. No adverse AZM effect was observed. Histology confirmed a significant reduction of infiltrating leukocytes. The short-term application of AZM for three days prior to transplantation or for seven days perioperatively reduced corneal graft rejection significantly compared with the controls. CONCLUSIONS: Along with antibiotic properties, topical AZM has a strong anti-inflammatory effect. Following keratoplasty, this effect is comparable to topical dexamethasone without the risk of steroid-induced adverse effects. Short-term treatment with AZM three days prior to the transplantation was sufficient to promote graft survival in the rat keratoplasty model. We therefore suggest further assessing the anti-inflammatory function of topical AZM following keratoplasty in humans.
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spelling pubmed-38572542013-12-13 Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat Wacker, Katrin Denker, Sophy Hildebrand, Antonia Eberwein, Philipp Reinhard, Thomas Schwartzkopff, Johannes PLoS One Research Article BACKGROUND: Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats. METHODS: Corneal transplants were performed between Fisher-donor and Lewis-recipient rats. Recipients were postoperatively treated three times daily with AZM, miglyol, ofloxacin or dexamethasone eye drops. As an additional control, AZM was applied following syngeneic keratoplasty. Furthermore, short-term treatments with AZM for seven days perioperatively or with AZM only three days prior to the transplantation were compared to appropriate controls. All transplants were monitored clinically for opacity, edema, and vascularization. Infiltrating CD45(+), CD4(+), CD8(+), CD25(+), CD161(+) and CD163(+) cells were quantified via immunohistochemistry. RESULTS: AZM significantly promoted corneal graft survival compared with miglyol or ofloxacin treatment. This effect was comparable to topical dexamethasone. No adverse AZM effect was observed. Histology confirmed a significant reduction of infiltrating leukocytes. The short-term application of AZM for three days prior to transplantation or for seven days perioperatively reduced corneal graft rejection significantly compared with the controls. CONCLUSIONS: Along with antibiotic properties, topical AZM has a strong anti-inflammatory effect. Following keratoplasty, this effect is comparable to topical dexamethasone without the risk of steroid-induced adverse effects. Short-term treatment with AZM three days prior to the transplantation was sufficient to promote graft survival in the rat keratoplasty model. We therefore suggest further assessing the anti-inflammatory function of topical AZM following keratoplasty in humans. Public Library of Science 2013-12-09 /pmc/articles/PMC3857254/ /pubmed/24349336 http://dx.doi.org/10.1371/journal.pone.0082687 Text en © 2013 Wacker et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wacker, Katrin
Denker, Sophy
Hildebrand, Antonia
Eberwein, Philipp
Reinhard, Thomas
Schwartzkopff, Johannes
Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
title Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
title_full Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
title_fullStr Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
title_full_unstemmed Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
title_short Short-Term Azithromycin Treatment Promotes Cornea Allograft Survival in the Rat
title_sort short-term azithromycin treatment promotes cornea allograft survival in the rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857254/
https://www.ncbi.nlm.nih.gov/pubmed/24349336
http://dx.doi.org/10.1371/journal.pone.0082687
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