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Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus sensu stricto Complex Based on Mitochondrial DNA Signature
The genetic diversity and population genetics of the Echinococcus granulosus sensu stricto complex were investigated based on sequencing of mitochondrial DNA (mtDNA). Total 81 isolates of hydatid cyst collected from ungulate animals from different geographical areas of North India were identified by...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857302/ https://www.ncbi.nlm.nih.gov/pubmed/24349394 http://dx.doi.org/10.1371/journal.pone.0082904 |
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author | Sharma, Monika Fomda, Bashir Ahmad Mazta, Saligram Sehgal, Rakesh Bagicha Singh, Balbir Malla, Nancy |
author_facet | Sharma, Monika Fomda, Bashir Ahmad Mazta, Saligram Sehgal, Rakesh Bagicha Singh, Balbir Malla, Nancy |
author_sort | Sharma, Monika |
collection | PubMed |
description | The genetic diversity and population genetics of the Echinococcus granulosus sensu stricto complex were investigated based on sequencing of mitochondrial DNA (mtDNA). Total 81 isolates of hydatid cyst collected from ungulate animals from different geographical areas of North India were identified by sequencing of cytochrome c oxidase subunit1 (coxi) gene. Three genotypes belonging to E. granulosus sensu stricto complex were identified (G1, G2 and G3 genotypes). Further the nucleotide sequences (retrieved from GenBank) for the coxi gene from seven populations of E. granulosus sensu stricto complex covering 6 continents, were compared with sequences of isolates analysed in this study. Molecular diversity indices represent overall high mitochondrial DNA diversity for these populations, but low nucleotide diversity between haplotypes. The neutrality tests were used to analyze signatures of historical demographic events. The Tajima’s D test and Fu’s FS test showed negative value, indicating deviations from neutrality and both suggested recent population expansion for the populations. Pairwise fixation index was significant for pairwise comparison of different populations (except between South America and East Asia, Middle East and Europe, South America and Europe, Africa and Australia), indicating genetic differentiation among populations. Based on the findings of the present study and those from earlier studies, we hypothesize that demographic expansion occurred in E. granulosus after the introduction of founder haplotype particular by anthropogenic movements. |
format | Online Article Text |
id | pubmed-3857302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38573022013-12-13 Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus sensu stricto Complex Based on Mitochondrial DNA Signature Sharma, Monika Fomda, Bashir Ahmad Mazta, Saligram Sehgal, Rakesh Bagicha Singh, Balbir Malla, Nancy PLoS One Research Article The genetic diversity and population genetics of the Echinococcus granulosus sensu stricto complex were investigated based on sequencing of mitochondrial DNA (mtDNA). Total 81 isolates of hydatid cyst collected from ungulate animals from different geographical areas of North India were identified by sequencing of cytochrome c oxidase subunit1 (coxi) gene. Three genotypes belonging to E. granulosus sensu stricto complex were identified (G1, G2 and G3 genotypes). Further the nucleotide sequences (retrieved from GenBank) for the coxi gene from seven populations of E. granulosus sensu stricto complex covering 6 continents, were compared with sequences of isolates analysed in this study. Molecular diversity indices represent overall high mitochondrial DNA diversity for these populations, but low nucleotide diversity between haplotypes. The neutrality tests were used to analyze signatures of historical demographic events. The Tajima’s D test and Fu’s FS test showed negative value, indicating deviations from neutrality and both suggested recent population expansion for the populations. Pairwise fixation index was significant for pairwise comparison of different populations (except between South America and East Asia, Middle East and Europe, South America and Europe, Africa and Australia), indicating genetic differentiation among populations. Based on the findings of the present study and those from earlier studies, we hypothesize that demographic expansion occurred in E. granulosus after the introduction of founder haplotype particular by anthropogenic movements. Public Library of Science 2013-12-09 /pmc/articles/PMC3857302/ /pubmed/24349394 http://dx.doi.org/10.1371/journal.pone.0082904 Text en © 2013 Sharma et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sharma, Monika Fomda, Bashir Ahmad Mazta, Saligram Sehgal, Rakesh Bagicha Singh, Balbir Malla, Nancy Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus sensu stricto Complex Based on Mitochondrial DNA Signature |
title | Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus
sensu stricto Complex Based on Mitochondrial DNA Signature |
title_full | Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus
sensu stricto Complex Based on Mitochondrial DNA Signature |
title_fullStr | Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus
sensu stricto Complex Based on Mitochondrial DNA Signature |
title_full_unstemmed | Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus
sensu stricto Complex Based on Mitochondrial DNA Signature |
title_short | Genetic Diversity and Population Genetic Structure Analysis of Echinococcus granulosus
sensu stricto Complex Based on Mitochondrial DNA Signature |
title_sort | genetic diversity and population genetic structure analysis of echinococcus granulosus
sensu stricto complex based on mitochondrial dna signature |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857302/ https://www.ncbi.nlm.nih.gov/pubmed/24349394 http://dx.doi.org/10.1371/journal.pone.0082904 |
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