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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein

Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic...

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Autores principales: Song, Kyoung Ju, Yang, Zhaoshou, Chong, Chom-Kyu, Kim, Jin-Soo, Lee, Kyung Chan, Kim, Tong-Soo, Nam, Ho-Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857496/
https://www.ncbi.nlm.nih.gov/pubmed/24327774
http://dx.doi.org/10.3347/kjp.2013.51.5.503
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author Song, Kyoung Ju
Yang, Zhaoshou
Chong, Chom-Kyu
Kim, Jin-Soo
Lee, Kyung Chan
Kim, Tong-Soo
Nam, Ho-Woo
author_facet Song, Kyoung Ju
Yang, Zhaoshou
Chong, Chom-Kyu
Kim, Jin-Soo
Lee, Kyung Chan
Kim, Tong-Soo
Nam, Ho-Woo
author_sort Song, Kyoung Ju
collection PubMed
description Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.
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spelling pubmed-38574962013-12-10 A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein Song, Kyoung Ju Yang, Zhaoshou Chong, Chom-Kyu Kim, Jin-Soo Lee, Kyung Chan Kim, Tong-Soo Nam, Ho-Woo Korean J Parasitol Original Article Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis. The Korean Society for Parasitology and Tropical Medicine 2013-10 2013-10-31 /pmc/articles/PMC3857496/ /pubmed/24327774 http://dx.doi.org/10.3347/kjp.2013.51.5.503 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Song, Kyoung Ju
Yang, Zhaoshou
Chong, Chom-Kyu
Kim, Jin-Soo
Lee, Kyung Chan
Kim, Tong-Soo
Nam, Ho-Woo
A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
title A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
title_full A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
title_fullStr A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
title_full_unstemmed A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
title_short A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
title_sort rapid diagnostic test for toxoplasmosis using recombinant antigenic n-terminal half of sag1 linked with intrinsically unstructured domain of gra2 protein
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857496/
https://www.ncbi.nlm.nih.gov/pubmed/24327774
http://dx.doi.org/10.3347/kjp.2013.51.5.503
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