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Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp
The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the mollus...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Parasitology and Tropical Medicine
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857501/ https://www.ncbi.nlm.nih.gov/pubmed/24327779 http://dx.doi.org/10.3347/kjp.2013.51.5.537 |
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author | Aukkanimart, Ratchadawan Boonmars, Thidarut Pinlaor, Somchai Tesana, Smarn Aunpromma, Surasit Booyarat, Chantana Sriraj, Pranee Laummaunwai, Porntip Punjaruk, Wiyada |
author_facet | Aukkanimart, Ratchadawan Boonmars, Thidarut Pinlaor, Somchai Tesana, Smarn Aunpromma, Surasit Booyarat, Chantana Sriraj, Pranee Laummaunwai, Porntip Punjaruk, Wiyada |
author_sort | Aukkanimart, Ratchadawan |
collection | PubMed |
description | The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration (LC(50)) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had LC(50) at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides. |
format | Online Article Text |
id | pubmed-3857501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Korean Society for Parasitology and Tropical Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-38575012013-12-10 Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp Aukkanimart, Ratchadawan Boonmars, Thidarut Pinlaor, Somchai Tesana, Smarn Aunpromma, Surasit Booyarat, Chantana Sriraj, Pranee Laummaunwai, Porntip Punjaruk, Wiyada Korean J Parasitol Original Article The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration (LC(50)) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had LC(50) at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides. The Korean Society for Parasitology and Tropical Medicine 2013-10 2013-10-31 /pmc/articles/PMC3857501/ /pubmed/24327779 http://dx.doi.org/10.3347/kjp.2013.51.5.537 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Aukkanimart, Ratchadawan Boonmars, Thidarut Pinlaor, Somchai Tesana, Smarn Aunpromma, Surasit Booyarat, Chantana Sriraj, Pranee Laummaunwai, Porntip Punjaruk, Wiyada Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp |
title | Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp |
title_full | Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp |
title_fullStr | Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp |
title_full_unstemmed | Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp |
title_short | Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp |
title_sort | histopathological changes in tissues of bithynia siamensis goniomphalos incubated in crude extracts of camellia seed and mangosteen pericarp |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857501/ https://www.ncbi.nlm.nih.gov/pubmed/24327779 http://dx.doi.org/10.3347/kjp.2013.51.5.537 |
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