Randomly broken fragment PCR with 5′ end-directed adaptor for genome walking

Many genome walking methods based on polymerase chain reaction (PCR) are available, including those with and without restriction enzyme modification. Nevertheless, these methods suffer from low reproducibility, inefficiency, and non-specificity. Here, we present a traceable and efficient PCR strateg...

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Detalles Bibliográficos
Autores principales: Xu, Wentao, Shang, Ying, Zhu, Pengyu, Zhai, Zhifang, He, Jing, Huang, Kunlun, Luo, Yunbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857568/
https://www.ncbi.nlm.nih.gov/pubmed/24322619
http://dx.doi.org/10.1038/srep03465

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857568/
https://www.ncbi.nlm.nih.gov/pubmed/24322619
http://dx.doi.org/10.1038/srep03465

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