Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells

Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific pep...

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Autores principales: Yoshioka, Yasukiyo, Ye, Yue Qi, Okada, Kiyoshi, Taniguchi, Kayoko, Yoshida, Ayaka, Sugaya, Kouichi, Onose, Jun-ichi, Koshino, Hiroyuki, Takahashi, Shunya, Yajima, Arata, Yajima, Shunsuke, Abe, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857809/
https://www.ncbi.nlm.nih.gov/pubmed/24349023
http://dx.doi.org/10.1371/journal.pone.0080931
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author Yoshioka, Yasukiyo
Ye, Yue Qi
Okada, Kiyoshi
Taniguchi, Kayoko
Yoshida, Ayaka
Sugaya, Kouichi
Onose, Jun-ichi
Koshino, Hiroyuki
Takahashi, Shunya
Yajima, Arata
Yajima, Shunsuke
Abe, Naoki
author_facet Yoshioka, Yasukiyo
Ye, Yue Qi
Okada, Kiyoshi
Taniguchi, Kayoko
Yoshida, Ayaka
Sugaya, Kouichi
Onose, Jun-ichi
Koshino, Hiroyuki
Takahashi, Shunya
Yajima, Arata
Yajima, Shunsuke
Abe, Naoki
author_sort Yoshioka, Yasukiyo
collection PubMed
description Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3.
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spelling pubmed-38578092013-12-12 Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells Yoshioka, Yasukiyo Ye, Yue Qi Okada, Kiyoshi Taniguchi, Kayoko Yoshida, Ayaka Sugaya, Kouichi Onose, Jun-ichi Koshino, Hiroyuki Takahashi, Shunya Yajima, Arata Yajima, Shunsuke Abe, Naoki PLoS One Research Article Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3. Public Library of Science 2013-12-04 /pmc/articles/PMC3857809/ /pubmed/24349023 http://dx.doi.org/10.1371/journal.pone.0080931 Text en © 2013 Yoshioka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yoshioka, Yasukiyo
Ye, Yue Qi
Okada, Kiyoshi
Taniguchi, Kayoko
Yoshida, Ayaka
Sugaya, Kouichi
Onose, Jun-ichi
Koshino, Hiroyuki
Takahashi, Shunya
Yajima, Arata
Yajima, Shunsuke
Abe, Naoki
Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells
title Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells
title_full Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells
title_fullStr Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells
title_full_unstemmed Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells
title_short Ubiquitin-Specific Peptidase 5, a Target Molecule of Vialinin A, Is a Key Molecule of TNF-α Production in RBL-2H3 Cells
title_sort ubiquitin-specific peptidase 5, a target molecule of vialinin a, is a key molecule of tnf-α production in rbl-2h3 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857809/
https://www.ncbi.nlm.nih.gov/pubmed/24349023
http://dx.doi.org/10.1371/journal.pone.0080931
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