Cargando…
Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores
The purpose of the present study is to examine the effects of essential oil of Citrus bergamia Risso (bergamot, BEO) on intracellular Ca(2+) in human umbilical vein endothelial cells. Fura-2 fluorescence was used to examine changes in intracellular Ca(2+) concentration [Ca(2+)](i) . In the presence...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857908/ https://www.ncbi.nlm.nih.gov/pubmed/24348719 http://dx.doi.org/10.1155/2013/759615 |
_version_ | 1782295218405507072 |
---|---|
author | Kang, Purum Han, Seung Ho Moon, Hea Kyung Lee, Jeong-Min Kim, Hyo-Keun Min, Sun Seek Seol, Geun Hee |
author_facet | Kang, Purum Han, Seung Ho Moon, Hea Kyung Lee, Jeong-Min Kim, Hyo-Keun Min, Sun Seek Seol, Geun Hee |
author_sort | Kang, Purum |
collection | PubMed |
description | The purpose of the present study is to examine the effects of essential oil of Citrus bergamia Risso (bergamot, BEO) on intracellular Ca(2+) in human umbilical vein endothelial cells. Fura-2 fluorescence was used to examine changes in intracellular Ca(2+) concentration [Ca(2+)](i) . In the presence of extracellular Ca(2+), BEO increased [Ca(2+)](i) , which was partially inhibited by a nonselective Ca(2+) channel blocker La(3+). In Ca(2+)-free extracellular solutions, BEO increased [Ca(2+)](i) in a concentration-dependent manner, suggesting that BEO mobilizes intracellular Ca(2+). BEO-induced [Ca(2+)](i) increase was partially inhibited by a Ca(2+)-induced Ca(2+) release inhibitor dantrolene, a phospholipase C inhibitor U73122, and an inositol 1,4,5-triphosphate (IP(3))-gated Ca(2+) channel blocker, 2-aminoethoxydiphenyl borane (2-APB). BEO also increased [Ca(2+)](i) in the presence of carbonyl cyanide m-chlorophenylhydrazone, an inhibitor of mitochondrial Ca(2+) uptake. In addition, store-operated Ca(2+) entry (SOC) was potentiated by BEO. These results suggest that BEO mobilizes Ca(2+) from primary intracellular stores via Ca(2+)-induced and IP3-mediated Ca(2+) release and affect promotion of Ca(2+) influx, likely via an SOC mechanism. |
format | Online Article Text |
id | pubmed-3857908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38579082013-12-17 Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores Kang, Purum Han, Seung Ho Moon, Hea Kyung Lee, Jeong-Min Kim, Hyo-Keun Min, Sun Seek Seol, Geun Hee Evid Based Complement Alternat Med Research Article The purpose of the present study is to examine the effects of essential oil of Citrus bergamia Risso (bergamot, BEO) on intracellular Ca(2+) in human umbilical vein endothelial cells. Fura-2 fluorescence was used to examine changes in intracellular Ca(2+) concentration [Ca(2+)](i) . In the presence of extracellular Ca(2+), BEO increased [Ca(2+)](i) , which was partially inhibited by a nonselective Ca(2+) channel blocker La(3+). In Ca(2+)-free extracellular solutions, BEO increased [Ca(2+)](i) in a concentration-dependent manner, suggesting that BEO mobilizes intracellular Ca(2+). BEO-induced [Ca(2+)](i) increase was partially inhibited by a Ca(2+)-induced Ca(2+) release inhibitor dantrolene, a phospholipase C inhibitor U73122, and an inositol 1,4,5-triphosphate (IP(3))-gated Ca(2+) channel blocker, 2-aminoethoxydiphenyl borane (2-APB). BEO also increased [Ca(2+)](i) in the presence of carbonyl cyanide m-chlorophenylhydrazone, an inhibitor of mitochondrial Ca(2+) uptake. In addition, store-operated Ca(2+) entry (SOC) was potentiated by BEO. These results suggest that BEO mobilizes Ca(2+) from primary intracellular stores via Ca(2+)-induced and IP3-mediated Ca(2+) release and affect promotion of Ca(2+) influx, likely via an SOC mechanism. Hindawi Publishing Corporation 2013 2013-11-21 /pmc/articles/PMC3857908/ /pubmed/24348719 http://dx.doi.org/10.1155/2013/759615 Text en Copyright © 2013 Purum Kang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kang, Purum Han, Seung Ho Moon, Hea Kyung Lee, Jeong-Min Kim, Hyo-Keun Min, Sun Seek Seol, Geun Hee Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores |
title |
Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores |
title_full |
Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores |
title_fullStr |
Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores |
title_full_unstemmed |
Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores |
title_short |
Citrus bergamia Risso Elevates Intracellular Ca(2+) in Human Vascular Endothelial Cells due to Release of Ca(2+) from Primary Intracellular Stores |
title_sort | citrus bergamia risso elevates intracellular ca(2+) in human vascular endothelial cells due to release of ca(2+) from primary intracellular stores |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857908/ https://www.ncbi.nlm.nih.gov/pubmed/24348719 http://dx.doi.org/10.1155/2013/759615 |
work_keys_str_mv | AT kangpurum citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores AT hanseungho citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores AT moonheakyung citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores AT leejeongmin citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores AT kimhyokeun citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores AT minsunseek citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores AT seolgeunhee citrusbergamiarissoelevatesintracellularca2inhumanvascularendothelialcellsduetoreleaseofca2fromprimaryintracellularstores |