Cargando…
NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro
Neuronal precursor cell-expressed developmentally down-regulated 4-1 (NEDD4-1) plays a great role in tumor cell growth, but its function and mechanism in cell invasive behavior are totally unknown. Here we report that NEDD4-1 regulates migration and invasion of malignant glioma cells via triggering...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858320/ https://www.ncbi.nlm.nih.gov/pubmed/24340059 http://dx.doi.org/10.1371/journal.pone.0082789 |
_version_ | 1782295268952113152 |
---|---|
author | Zhang, Hao Nie, Wenchen Zhang, Xu Zhang, Gentang Li, Zhiqiang Wu, Huaibing Shi, Qiong Chen, Yong Ding, Zhijun Zhou, Xiuping Yu, Rutong |
author_facet | Zhang, Hao Nie, Wenchen Zhang, Xu Zhang, Gentang Li, Zhiqiang Wu, Huaibing Shi, Qiong Chen, Yong Ding, Zhijun Zhou, Xiuping Yu, Rutong |
author_sort | Zhang, Hao |
collection | PubMed |
description | Neuronal precursor cell-expressed developmentally down-regulated 4-1 (NEDD4-1) plays a great role in tumor cell growth, but its function and mechanism in cell invasive behavior are totally unknown. Here we report that NEDD4-1 regulates migration and invasion of malignant glioma cells via triggering ubiquitination of cyclic nucleotide Ras guanine nucleotide exchange factor (CNrasGEF) using cultured glioma cells. NEDD4-1 overexpression promoted cell migration and invasion, while its downregulation specifically inhibited them. However, NEDD4-1 did not affect the proliferation and apoptosis of glioma cells. NEDD4-1 physically interacted with CNrasGEF and promoted its poly-ubiquitination and degradation. Contrary to the effect of NEDD4-1, CNrasGEF downregulation promoted cell migration and invasion, while its overexpression inhibited them. Importantly, downregulation of CNrasGEF facilitated the effect of NEDD4-1-induced cell migration and invasion. Interestingly, aberrant up-regulated NEDD4-1 showed reverse correlation with CNrasGEF protein level but not with its mRNA level in glioma tissues. Combined with the in vitro results, the result of glioma tissues indicated post-translationally modification effect of NEDD4-1 on CNrasGEF. Our study suggests that NEDD4-1 regulates cell migration and invasion through ubiquitination of CNrasGEF in vitro. |
format | Online Article Text |
id | pubmed-3858320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38583202013-12-11 NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro Zhang, Hao Nie, Wenchen Zhang, Xu Zhang, Gentang Li, Zhiqiang Wu, Huaibing Shi, Qiong Chen, Yong Ding, Zhijun Zhou, Xiuping Yu, Rutong PLoS One Research Article Neuronal precursor cell-expressed developmentally down-regulated 4-1 (NEDD4-1) plays a great role in tumor cell growth, but its function and mechanism in cell invasive behavior are totally unknown. Here we report that NEDD4-1 regulates migration and invasion of malignant glioma cells via triggering ubiquitination of cyclic nucleotide Ras guanine nucleotide exchange factor (CNrasGEF) using cultured glioma cells. NEDD4-1 overexpression promoted cell migration and invasion, while its downregulation specifically inhibited them. However, NEDD4-1 did not affect the proliferation and apoptosis of glioma cells. NEDD4-1 physically interacted with CNrasGEF and promoted its poly-ubiquitination and degradation. Contrary to the effect of NEDD4-1, CNrasGEF downregulation promoted cell migration and invasion, while its overexpression inhibited them. Importantly, downregulation of CNrasGEF facilitated the effect of NEDD4-1-induced cell migration and invasion. Interestingly, aberrant up-regulated NEDD4-1 showed reverse correlation with CNrasGEF protein level but not with its mRNA level in glioma tissues. Combined with the in vitro results, the result of glioma tissues indicated post-translationally modification effect of NEDD4-1 on CNrasGEF. Our study suggests that NEDD4-1 regulates cell migration and invasion through ubiquitination of CNrasGEF in vitro. Public Library of Science 2013-12-10 /pmc/articles/PMC3858320/ /pubmed/24340059 http://dx.doi.org/10.1371/journal.pone.0082789 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Hao Nie, Wenchen Zhang, Xu Zhang, Gentang Li, Zhiqiang Wu, Huaibing Shi, Qiong Chen, Yong Ding, Zhijun Zhou, Xiuping Yu, Rutong NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro |
title | NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro |
title_full | NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro |
title_fullStr | NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro |
title_full_unstemmed | NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro |
title_short | NEDD4-1 Regulates Migration and Invasion of Glioma Cells through CNrasGEF Ubiquitination In Vitro |
title_sort | nedd4-1 regulates migration and invasion of glioma cells through cnrasgef ubiquitination in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858320/ https://www.ncbi.nlm.nih.gov/pubmed/24340059 http://dx.doi.org/10.1371/journal.pone.0082789 |
work_keys_str_mv | AT zhanghao nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT niewenchen nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT zhangxu nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT zhanggentang nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT lizhiqiang nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT wuhuaibing nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT shiqiong nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT chenyong nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT dingzhijun nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT zhouxiuping nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro AT yurutong nedd41regulatesmigrationandinvasionofgliomacellsthroughcnrasgefubiquitinationinvitro |