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Development of a Rapid Streptavidin Capture-Based Assay for the Tyrosine Phosphorylated CSF-1R in Peripheral Blood Mononuclear Cells

A novel assay was developed to measure ratio of p-FMS (phospho FMS) to FMS using the Meso Scale Discovery(®) (MSD) technology and compared to the routinely used, IP-Western based approach. The existing IP-Western assay used lysed PBMCs (Peripheral Blood Mononuclear Cells) that were immunoprecipitate...

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Detalles Bibliográficos
Autores principales:	Chaturvedi, Shalini, Dell, Elayne, Siegel, Derick, Brittingham, Gregory, Seetharam, Shobha
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Ivyspring International Publisher 2013
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858583/ https://www.ncbi.nlm.nih.gov/pubmed/24339731 http://dx.doi.org/10.7150/ijbs.7268

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