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Matrix identity and tractional forces influence indirect cardiac reprogramming
Heart regeneration through in vivo cardiac reprogramming has been demonstrated as a possible regenerative strategy. While it has been reported that cardiac reprogramming in vivo is more efficient than in vitro, the influence of the extracellular microenvironment on cardiac reprogramming remains inco...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858798/ https://www.ncbi.nlm.nih.gov/pubmed/24326998 http://dx.doi.org/10.1038/srep03474 |
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author | Kong, Yen P. Carrion, Bita Singh, Rahul K. Putnam, Andrew J. |
author_facet | Kong, Yen P. Carrion, Bita Singh, Rahul K. Putnam, Andrew J. |
author_sort | Kong, Yen P. |
collection | PubMed |
description | Heart regeneration through in vivo cardiac reprogramming has been demonstrated as a possible regenerative strategy. While it has been reported that cardiac reprogramming in vivo is more efficient than in vitro, the influence of the extracellular microenvironment on cardiac reprogramming remains incompletely understood. This understanding is necessary to improve the efficiency of cardiac reprogramming in order to implement this strategy successfully. Here we have identified matrix identity and cell-generated tractional forces as key determinants of the dedifferentiation and differentiation stages during reprogramming. Cell proliferation, matrix mechanics, and matrix microstructure are also important, but play lesser roles. Our results suggest that the extracellular microenvironment can be optimized to enhance cardiac reprogramming. |
format | Online Article Text |
id | pubmed-3858798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-38587982013-12-11 Matrix identity and tractional forces influence indirect cardiac reprogramming Kong, Yen P. Carrion, Bita Singh, Rahul K. Putnam, Andrew J. Sci Rep Article Heart regeneration through in vivo cardiac reprogramming has been demonstrated as a possible regenerative strategy. While it has been reported that cardiac reprogramming in vivo is more efficient than in vitro, the influence of the extracellular microenvironment on cardiac reprogramming remains incompletely understood. This understanding is necessary to improve the efficiency of cardiac reprogramming in order to implement this strategy successfully. Here we have identified matrix identity and cell-generated tractional forces as key determinants of the dedifferentiation and differentiation stages during reprogramming. Cell proliferation, matrix mechanics, and matrix microstructure are also important, but play lesser roles. Our results suggest that the extracellular microenvironment can be optimized to enhance cardiac reprogramming. Nature Publishing Group 2013-12-11 /pmc/articles/PMC3858798/ /pubmed/24326998 http://dx.doi.org/10.1038/srep03474 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Kong, Yen P. Carrion, Bita Singh, Rahul K. Putnam, Andrew J. Matrix identity and tractional forces influence indirect cardiac reprogramming |
title | Matrix identity and tractional forces influence indirect cardiac reprogramming |
title_full | Matrix identity and tractional forces influence indirect cardiac reprogramming |
title_fullStr | Matrix identity and tractional forces influence indirect cardiac reprogramming |
title_full_unstemmed | Matrix identity and tractional forces influence indirect cardiac reprogramming |
title_short | Matrix identity and tractional forces influence indirect cardiac reprogramming |
title_sort | matrix identity and tractional forces influence indirect cardiac reprogramming |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858798/ https://www.ncbi.nlm.nih.gov/pubmed/24326998 http://dx.doi.org/10.1038/srep03474 |
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