Cargando…
Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria
This study compares the performance of clinical diagnosis and three laboratory diagnostic methods (thick film microscopy (TFM), rapid diagnostic test (RDT), and polymerase chain reaction (PCR)) for the diagnosis of Plasmodium falciparum in Nigeria. Using clinical criteria, 217 children were recruite...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859170/ https://www.ncbi.nlm.nih.gov/pubmed/24371538 http://dx.doi.org/10.1155/2013/308069 |
_version_ | 1782295388770795520 |
---|---|
author | Ojurongbe, Olusola Adegbosin, Olunike Olayeni Taiwo, Sunday Samuel Alli, Oyebode Armstrong Terry Olowe, Olugbenga Adekunle Ojurongbe, Taiwo Adetola Bolaji, Oloyede Samuel Adeyeba, Oluwaseyi Adegboyega |
author_facet | Ojurongbe, Olusola Adegbosin, Olunike Olayeni Taiwo, Sunday Samuel Alli, Oyebode Armstrong Terry Olowe, Olugbenga Adekunle Ojurongbe, Taiwo Adetola Bolaji, Oloyede Samuel Adeyeba, Oluwaseyi Adegboyega |
author_sort | Ojurongbe, Olusola |
collection | PubMed |
description | This study compares the performance of clinical diagnosis and three laboratory diagnostic methods (thick film microscopy (TFM), rapid diagnostic test (RDT), and polymerase chain reaction (PCR)) for the diagnosis of Plasmodium falciparum in Nigeria. Using clinical criteria, 217 children were recruited into the study out of which 106 (48.8%) were positive by TFM, 84 (38.7%) by RDT, and 125 (57.6%) by PCR. Using a composite reference method generated from the three diagnostic methods, 71 (32.7%) patients were found to be truly infected and 90 (41.5%) truly uninfected, while 56 (25.8%) were misidentified as infected or noninfected. When each of the 3 diagnostic methods was compared with the composite reference, PCR had sensitivity of 97.3%, specificity of 62.5%, positive predictive value (PPV) of 56.8%, and negative predictive value (NPV) of 97.8%; microscopy had sensitivity of 77.2%, specificity of 72%, PPV of 66.9%, and NPV of 81.1%, while RDT had sensitivity of 62.3%, specificity of 87.4%, PPV of 67.7%, and NPV of 84.5%. PCR test performed best among the three methods followed by TFM and RDT in that order. The result of this study shows that clinical diagnosis cannot be relied upon for accurate diagnosis of P. falciparum in endemic areas. |
format | Online Article Text |
id | pubmed-3859170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38591702013-12-26 Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria Ojurongbe, Olusola Adegbosin, Olunike Olayeni Taiwo, Sunday Samuel Alli, Oyebode Armstrong Terry Olowe, Olugbenga Adekunle Ojurongbe, Taiwo Adetola Bolaji, Oloyede Samuel Adeyeba, Oluwaseyi Adegboyega Malar Res Treat Research Article This study compares the performance of clinical diagnosis and three laboratory diagnostic methods (thick film microscopy (TFM), rapid diagnostic test (RDT), and polymerase chain reaction (PCR)) for the diagnosis of Plasmodium falciparum in Nigeria. Using clinical criteria, 217 children were recruited into the study out of which 106 (48.8%) were positive by TFM, 84 (38.7%) by RDT, and 125 (57.6%) by PCR. Using a composite reference method generated from the three diagnostic methods, 71 (32.7%) patients were found to be truly infected and 90 (41.5%) truly uninfected, while 56 (25.8%) were misidentified as infected or noninfected. When each of the 3 diagnostic methods was compared with the composite reference, PCR had sensitivity of 97.3%, specificity of 62.5%, positive predictive value (PPV) of 56.8%, and negative predictive value (NPV) of 97.8%; microscopy had sensitivity of 77.2%, specificity of 72%, PPV of 66.9%, and NPV of 81.1%, while RDT had sensitivity of 62.3%, specificity of 87.4%, PPV of 67.7%, and NPV of 84.5%. PCR test performed best among the three methods followed by TFM and RDT in that order. The result of this study shows that clinical diagnosis cannot be relied upon for accurate diagnosis of P. falciparum in endemic areas. Hindawi Publishing Corporation 2013 2013-11-24 /pmc/articles/PMC3859170/ /pubmed/24371538 http://dx.doi.org/10.1155/2013/308069 Text en Copyright © 2013 Olusola Ojurongbe et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ojurongbe, Olusola Adegbosin, Olunike Olayeni Taiwo, Sunday Samuel Alli, Oyebode Armstrong Terry Olowe, Olugbenga Adekunle Ojurongbe, Taiwo Adetola Bolaji, Oloyede Samuel Adeyeba, Oluwaseyi Adegboyega Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria |
title | Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria |
title_full | Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria |
title_fullStr | Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria |
title_full_unstemmed | Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria |
title_short | Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria |
title_sort | assessment of clinical diagnosis, microscopy, rapid diagnostic tests, and polymerase chain reaction in the diagnosis of plasmodium falciparum in nigeria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859170/ https://www.ncbi.nlm.nih.gov/pubmed/24371538 http://dx.doi.org/10.1155/2013/308069 |
work_keys_str_mv | AT ojurongbeolusola assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT adegbosinolunikeolayeni assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT taiwosundaysamuel assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT allioyebodearmstrongterry assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT oloweolugbengaadekunle assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT ojurongbetaiwoadetola assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT bolajioloyedesamuel assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria AT adeyebaoluwaseyiadegboyega assessmentofclinicaldiagnosismicroscopyrapiddiagnostictestsandpolymerasechainreactioninthediagnosisofplasmodiumfalciparuminnigeria |