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An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison

Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. It is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. The genes, proteins and metabolites tha...

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Autores principales: Terrasson, Emmanuel, Buitink, Julia, Righetti, Karima, Ly Vu, Benoit, Pelletier, Sandra, Zinsmeister, Julia, Lalanne, David, Leprince, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859232/
https://www.ncbi.nlm.nih.gov/pubmed/24376450
http://dx.doi.org/10.3389/fpls.2013.00497
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author Terrasson, Emmanuel
Buitink, Julia
Righetti, Karima
Ly Vu, Benoit
Pelletier, Sandra
Zinsmeister, Julia
Lalanne, David
Leprince, Olivier
author_facet Terrasson, Emmanuel
Buitink, Julia
Righetti, Karima
Ly Vu, Benoit
Pelletier, Sandra
Zinsmeister, Julia
Lalanne, David
Leprince, Olivier
author_sort Terrasson, Emmanuel
collection PubMed
description Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. It is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. The genes, proteins and metabolites that are required to establish this DT is referred to as the desiccome. It includes both a range of protective mechanisms and underlying regulatory pathways that remain poorly understood. As a first step toward the identification of the seed desiccome of Medicago truncatula, using updated microarrays we characterized the overlapping transcriptomes associated with acquisition of DT in developing seeds and the re-establishment of DT in germinated seeds using a polyethylene glycol treatment (−1.7 MPa). The resulting list contained 740 and 2829 transcripts whose levels, respectively, increased and decreased with DT. Fourty-eight transcription factors (TF) were identified including MtABI3, MtABI5 and many genes regulating flowering transition and cell identity. A promoter enrichment analysis revealed a strong over-representation of ABRE elements together with light-responsive cis-acting elements. In Mtabi5 Tnt1 insertion mutants, DT could no longer be re-established by an osmotic stress. Transcriptome analysis on Mtabi5 radicles during osmotic stress revealed that 13 and 15% of the up-regulated and down-regulated genes, respectively, are mis-regulated in the mutants and might be putative downstream targets of MtABI5 implicated in the re-establishment of DT. Likewise, transcriptome comparisons of the desiccation sensitive Mtabi3 mutants and hairy roots ectopically expressing MtABI3 revealed that 35 and 23% of the up-regulated and down-regulated genes are acting downstream of MtABI3. Our data suggest that ABI3 and ABI5 have complementary roles in DT. Whether DT evolved by co-opting existing pathways regulating flowering and cellular phase transition and cell identity is discussed.
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spelling pubmed-38592322013-12-27 An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison Terrasson, Emmanuel Buitink, Julia Righetti, Karima Ly Vu, Benoit Pelletier, Sandra Zinsmeister, Julia Lalanne, David Leprince, Olivier Front Plant Sci Plant Science Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. It is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. The genes, proteins and metabolites that are required to establish this DT is referred to as the desiccome. It includes both a range of protective mechanisms and underlying regulatory pathways that remain poorly understood. As a first step toward the identification of the seed desiccome of Medicago truncatula, using updated microarrays we characterized the overlapping transcriptomes associated with acquisition of DT in developing seeds and the re-establishment of DT in germinated seeds using a polyethylene glycol treatment (−1.7 MPa). The resulting list contained 740 and 2829 transcripts whose levels, respectively, increased and decreased with DT. Fourty-eight transcription factors (TF) were identified including MtABI3, MtABI5 and many genes regulating flowering transition and cell identity. A promoter enrichment analysis revealed a strong over-representation of ABRE elements together with light-responsive cis-acting elements. In Mtabi5 Tnt1 insertion mutants, DT could no longer be re-established by an osmotic stress. Transcriptome analysis on Mtabi5 radicles during osmotic stress revealed that 13 and 15% of the up-regulated and down-regulated genes, respectively, are mis-regulated in the mutants and might be putative downstream targets of MtABI5 implicated in the re-establishment of DT. Likewise, transcriptome comparisons of the desiccation sensitive Mtabi3 mutants and hairy roots ectopically expressing MtABI3 revealed that 35 and 23% of the up-regulated and down-regulated genes are acting downstream of MtABI3. Our data suggest that ABI3 and ABI5 have complementary roles in DT. Whether DT evolved by co-opting existing pathways regulating flowering and cellular phase transition and cell identity is discussed. Frontiers Media S.A. 2013-12-11 /pmc/articles/PMC3859232/ /pubmed/24376450 http://dx.doi.org/10.3389/fpls.2013.00497 Text en Copyright © 2013 Terrasson, Buitink, Righetti, Ly Vu, Pelletier, Zinsmeister, Lalanne and Leprince. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Terrasson, Emmanuel
Buitink, Julia
Righetti, Karima
Ly Vu, Benoit
Pelletier, Sandra
Zinsmeister, Julia
Lalanne, David
Leprince, Olivier
An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
title An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
title_full An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
title_fullStr An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
title_full_unstemmed An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
title_short An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
title_sort emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859232/
https://www.ncbi.nlm.nih.gov/pubmed/24376450
http://dx.doi.org/10.3389/fpls.2013.00497
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