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Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats
The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860984/ https://www.ncbi.nlm.nih.gov/pubmed/24348794 http://dx.doi.org/10.3892/etm.2013.1396 |
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author | YE, FEI CHEN, XU-QIN BAO, GUAN-SHUI HUA, YIN WANG, ZHE-DONG BAO, YI-CHUAN |
author_facet | YE, FEI CHEN, XU-QIN BAO, GUAN-SHUI HUA, YIN WANG, ZHE-DONG BAO, YI-CHUAN |
author_sort | YE, FEI |
collection | PubMed |
description | The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected to chronic electrical stimulation through the electrodes to induce kindling. The seizure characteristics and behavioral changes of the rats were observed, and electroencephalograms were recorded during and following kindling. The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared. The mean time-period required for kindling was 13.50±3.99 days, and the afterdischarge duration (ADD) measured between 21,450 and 119,720 msec. The expression of IL-6 mRNA was significantly upregulated in the kindled rats. TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats. In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling. |
format | Online Article Text |
id | pubmed-3860984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-38609842013-12-13 Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats YE, FEI CHEN, XU-QIN BAO, GUAN-SHUI HUA, YIN WANG, ZHE-DONG BAO, YI-CHUAN Exp Ther Med Articles The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected to chronic electrical stimulation through the electrodes to induce kindling. The seizure characteristics and behavioral changes of the rats were observed, and electroencephalograms were recorded during and following kindling. The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared. The mean time-period required for kindling was 13.50±3.99 days, and the afterdischarge duration (ADD) measured between 21,450 and 119,720 msec. The expression of IL-6 mRNA was significantly upregulated in the kindled rats. TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats. In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling. D.A. Spandidos 2014-01 2013-11-08 /pmc/articles/PMC3860984/ /pubmed/24348794 http://dx.doi.org/10.3892/etm.2013.1396 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles YE, FEI CHEN, XU-QIN BAO, GUAN-SHUI HUA, YIN WANG, ZHE-DONG BAO, YI-CHUAN Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
title | Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
title_full | Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
title_fullStr | Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
title_full_unstemmed | Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
title_short | Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
title_sort | effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860984/ https://www.ncbi.nlm.nih.gov/pubmed/24348794 http://dx.doi.org/10.3892/etm.2013.1396 |
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