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Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts

In migrating fibroblasts, rearward movement of the nucleus orients the centrosome toward the leading edge. Nuclear movement results from coupling rearward-moving, dorsal actin cables to the nucleus by linear arrays of nesprin-2G and SUN2, termed transmembrane actin-associated nuclear (TAN) lines. A-...

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Autores principales: Chang, Wakam, Folker, Eric S., Worman, Howard J., Gundersen, Gregg G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861083/
https://www.ncbi.nlm.nih.gov/pubmed/24152738
http://dx.doi.org/10.1091/mbc.E13-06-0307
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author Chang, Wakam
Folker, Eric S.
Worman, Howard J.
Gundersen, Gregg G.
author_facet Chang, Wakam
Folker, Eric S.
Worman, Howard J.
Gundersen, Gregg G.
author_sort Chang, Wakam
collection PubMed
description In migrating fibroblasts, rearward movement of the nucleus orients the centrosome toward the leading edge. Nuclear movement results from coupling rearward-moving, dorsal actin cables to the nucleus by linear arrays of nesprin-2G and SUN2, termed transmembrane actin-associated nuclear (TAN) lines. A-type lamins anchor TAN lines, prompting us to test whether emerin, a nuclear membrane protein that interacts with lamins and TAN line proteins, contributes to nuclear movement. In fibroblasts depleted of emerin, nuclei moved nondirectionally or completely failed to move. Consistent with these nuclear movement defects, dorsal actin cable flow was nondirectional in cells lacking emerin. TAN lines formed normally in cells lacking emerin and were coordinated with the erratic nuclear movements, although in 20% of the cases, TAN lines slipped over immobile nuclei. Myosin II drives actin flow, and depletion of myosin IIB, but not myosin IIA, showed similar nondirectional nuclear movement and actin flow as in emerin-depleted cells. Myosin IIB specifically coimmunoprecipitated with emerin, and emerin depletion prevented myosin IIB localization near nuclei. These results show that emerin functions with myosin IIB to polarize actin flow and nuclear movement in fibroblasts, suggesting a novel function for the nuclear envelope in organizing directional actin flow and cytoplasmic polarity.
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spelling pubmed-38610832014-03-02 Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts Chang, Wakam Folker, Eric S. Worman, Howard J. Gundersen, Gregg G. Mol Biol Cell Articles In migrating fibroblasts, rearward movement of the nucleus orients the centrosome toward the leading edge. Nuclear movement results from coupling rearward-moving, dorsal actin cables to the nucleus by linear arrays of nesprin-2G and SUN2, termed transmembrane actin-associated nuclear (TAN) lines. A-type lamins anchor TAN lines, prompting us to test whether emerin, a nuclear membrane protein that interacts with lamins and TAN line proteins, contributes to nuclear movement. In fibroblasts depleted of emerin, nuclei moved nondirectionally or completely failed to move. Consistent with these nuclear movement defects, dorsal actin cable flow was nondirectional in cells lacking emerin. TAN lines formed normally in cells lacking emerin and were coordinated with the erratic nuclear movements, although in 20% of the cases, TAN lines slipped over immobile nuclei. Myosin II drives actin flow, and depletion of myosin IIB, but not myosin IIA, showed similar nondirectional nuclear movement and actin flow as in emerin-depleted cells. Myosin IIB specifically coimmunoprecipitated with emerin, and emerin depletion prevented myosin IIB localization near nuclei. These results show that emerin functions with myosin IIB to polarize actin flow and nuclear movement in fibroblasts, suggesting a novel function for the nuclear envelope in organizing directional actin flow and cytoplasmic polarity. The American Society for Cell Biology 2013-12-15 /pmc/articles/PMC3861083/ /pubmed/24152738 http://dx.doi.org/10.1091/mbc.E13-06-0307 Text en © 2013 Chang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Chang, Wakam
Folker, Eric S.
Worman, Howard J.
Gundersen, Gregg G.
Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
title Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
title_full Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
title_fullStr Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
title_full_unstemmed Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
title_short Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
title_sort emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861083/
https://www.ncbi.nlm.nih.gov/pubmed/24152738
http://dx.doi.org/10.1091/mbc.E13-06-0307
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