Efficient Isolation of Pure and Functional Mitochondria from Mouse Tissues Using Automated Tissue Disruption and Enrichment with Anti-TOM22 Magnetic Beads

To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation result in isolates of highly variable quality...

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Detalles Bibliográficos
Autores principales: Franko, Andras, Baris, Olivier R., Bergschneider, Eva, von Toerne, Christine, Hauck, Stefanie M., Aichler, Michaela, Walch, Axel K., Wurst, Wolfgang, Wiesner, Rudolf J., Johnston, Ian C. D., de Angelis, Martin Hrabĕ
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861405/
https://www.ncbi.nlm.nih.gov/pubmed/24349272
http://dx.doi.org/10.1371/journal.pone.0082392

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