Cargando…
Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro
Valproic acid (VPA) is a well-tolerated drug that is used to treat seizure disorders and that has recently been shown to inhibit histone deacetylase. The present study investigated the effects of VPA on the radiosensitization of the rat C6 glioma cell line in vitro. To select an appropriate treatmen...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861595/ https://www.ncbi.nlm.nih.gov/pubmed/24348849 http://dx.doi.org/10.3892/ol.2013.1666 |
_version_ | 1782295671633608704 |
---|---|
author | ZHOU, YONG XU, YING WANG, HAN NIU, JUNJIE HOU, HUAYING JIANG, YUHUA |
author_facet | ZHOU, YONG XU, YING WANG, HAN NIU, JUNJIE HOU, HUAYING JIANG, YUHUA |
author_sort | ZHOU, YONG |
collection | PubMed |
description | Valproic acid (VPA) is a well-tolerated drug that is used to treat seizure disorders and that has recently been shown to inhibit histone deacetylase. The present study investigated the effects of VPA on the radiosensitization of the rat C6 glioma cell line in vitro. To select an appropriate treatment concentration and time, MTT and flow cytometry assays were performed to measure the inhibitory effects of VPA at various concentrations and incubation time-points. The radiosensitizing effect of VPA was determined using clonogenic experiments. VPA- and radiation-induced C6 apoptosis was analyzed using quantitative polymerase chain reaction and western blot analysis. Cell proliferation was significantly inhibited by VPA in a time- and dose-dependent manner (P<0.05). VPA enhanced radiation-induced C6 cell death and there was clear inhibition of clonogenic formation [sensitizer enhancement ratio (SER), 1.30]. This effect was closely associated with the concentration of VPA. VPA treatment decreased the mRNA and protein levels of Bcl-2, whereas increased changes were detected with Bax. At a concentration of 0.5 mmol/l, VPA had a low toxicity and enhanced the radiosensitization of the C6 cells. VPA may radiosensitize glioma cells by inhibiting cellular proliferation and inducing apoptosis by regulating apoptosis-related molecular changes. |
format | Online Article Text |
id | pubmed-3861595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-38615952013-12-13 Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro ZHOU, YONG XU, YING WANG, HAN NIU, JUNJIE HOU, HUAYING JIANG, YUHUA Oncol Lett Articles Valproic acid (VPA) is a well-tolerated drug that is used to treat seizure disorders and that has recently been shown to inhibit histone deacetylase. The present study investigated the effects of VPA on the radiosensitization of the rat C6 glioma cell line in vitro. To select an appropriate treatment concentration and time, MTT and flow cytometry assays were performed to measure the inhibitory effects of VPA at various concentrations and incubation time-points. The radiosensitizing effect of VPA was determined using clonogenic experiments. VPA- and radiation-induced C6 apoptosis was analyzed using quantitative polymerase chain reaction and western blot analysis. Cell proliferation was significantly inhibited by VPA in a time- and dose-dependent manner (P<0.05). VPA enhanced radiation-induced C6 cell death and there was clear inhibition of clonogenic formation [sensitizer enhancement ratio (SER), 1.30]. This effect was closely associated with the concentration of VPA. VPA treatment decreased the mRNA and protein levels of Bcl-2, whereas increased changes were detected with Bax. At a concentration of 0.5 mmol/l, VPA had a low toxicity and enhanced the radiosensitization of the C6 cells. VPA may radiosensitize glioma cells by inhibiting cellular proliferation and inducing apoptosis by regulating apoptosis-related molecular changes. D.A. Spandidos 2014-01 2013-11-07 /pmc/articles/PMC3861595/ /pubmed/24348849 http://dx.doi.org/10.3892/ol.2013.1666 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles ZHOU, YONG XU, YING WANG, HAN NIU, JUNJIE HOU, HUAYING JIANG, YUHUA Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro |
title | Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro |
title_full | Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro |
title_fullStr | Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro |
title_full_unstemmed | Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro |
title_short | Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro |
title_sort | histone deacetylase inhibitor, valproic acid, radiosensitizes the c6 glioma cell line in vitro |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861595/ https://www.ncbi.nlm.nih.gov/pubmed/24348849 http://dx.doi.org/10.3892/ol.2013.1666 |
work_keys_str_mv | AT zhouyong histonedeacetylaseinhibitorvalproicacidradiosensitizesthec6gliomacelllineinvitro AT xuying histonedeacetylaseinhibitorvalproicacidradiosensitizesthec6gliomacelllineinvitro AT wanghan histonedeacetylaseinhibitorvalproicacidradiosensitizesthec6gliomacelllineinvitro AT niujunjie histonedeacetylaseinhibitorvalproicacidradiosensitizesthec6gliomacelllineinvitro AT houhuaying histonedeacetylaseinhibitorvalproicacidradiosensitizesthec6gliomacelllineinvitro AT jiangyuhua histonedeacetylaseinhibitorvalproicacidradiosensitizesthec6gliomacelllineinvitro |