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The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress
In order to study the role of PIP1 aquaporins in leaf water and CO(2) transport, several lines of PIP1-deficient transgenic Populus tremula x alba were generated using a reverse genetic approach. These transgenic lines displayed no visible developmental or morphological phenotypes when grown under c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861612/ https://www.ncbi.nlm.nih.gov/pubmed/24379822 http://dx.doi.org/10.3389/fpls.2013.00507 |
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author | Secchi, Francesca Zwieniecki, Maciej A. |
author_facet | Secchi, Francesca Zwieniecki, Maciej A. |
author_sort | Secchi, Francesca |
collection | PubMed |
description | In order to study the role of PIP1 aquaporins in leaf water and CO(2) transport, several lines of PIP1-deficient transgenic Populus tremula x alba were generated using a reverse genetic approach. These transgenic lines displayed no visible developmental or morphological phenotypes when grown under conditions of no water stress. Major photosynthetic parameters were also not affected by PIP1 down regulation. However, low levels of PIP1 expression resulted in greater leaf hydraulic resistance (an increase of 27%), which effectively implicated PIP1 role in water transport. Additionally, the expression level of PIP1 genes in the various transgenic lines was correlated with reductions in mesophyll conductance to CO(2) (g(m)), suggesting that in poplar, these aquaporins influenced membrane permeability to CO(2). Overall, although analysis showed that PIP1 genes contributed to the mass transfer of water and CO(2) in poplar leaves, their down-regulation did not dramatically impair the physiological needs of this fast growing tree when cultivated under conditions of no stress. |
format | Online Article Text |
id | pubmed-3861612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-38616122013-12-30 The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress Secchi, Francesca Zwieniecki, Maciej A. Front Plant Sci Plant Science In order to study the role of PIP1 aquaporins in leaf water and CO(2) transport, several lines of PIP1-deficient transgenic Populus tremula x alba were generated using a reverse genetic approach. These transgenic lines displayed no visible developmental or morphological phenotypes when grown under conditions of no water stress. Major photosynthetic parameters were also not affected by PIP1 down regulation. However, low levels of PIP1 expression resulted in greater leaf hydraulic resistance (an increase of 27%), which effectively implicated PIP1 role in water transport. Additionally, the expression level of PIP1 genes in the various transgenic lines was correlated with reductions in mesophyll conductance to CO(2) (g(m)), suggesting that in poplar, these aquaporins influenced membrane permeability to CO(2). Overall, although analysis showed that PIP1 genes contributed to the mass transfer of water and CO(2) in poplar leaves, their down-regulation did not dramatically impair the physiological needs of this fast growing tree when cultivated under conditions of no stress. Frontiers Media S.A. 2013-12-13 /pmc/articles/PMC3861612/ /pubmed/24379822 http://dx.doi.org/10.3389/fpls.2013.00507 Text en Copyright © 2013 Secchi and Zwieniecki. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Secchi, Francesca Zwieniecki, Maciej A. The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress |
title | The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress |
title_full | The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress |
title_fullStr | The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress |
title_full_unstemmed | The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress |
title_short | The physiological response of Populus tremula x alba leaves to the down-regulation of PIP1 aquaporin gene expression under no water stress |
title_sort | physiological response of populus tremula x alba leaves to the down-regulation of pip1 aquaporin gene expression under no water stress |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861612/ https://www.ncbi.nlm.nih.gov/pubmed/24379822 http://dx.doi.org/10.3389/fpls.2013.00507 |
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