Cargando…

Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps

OBJECTIVE: To assess the regeneration of osteochondral defects in the joint cartilage of the knee induced by autologous platelet-rich plasma (PRP). METHODS: Osteochondral defects produced in the trochlear groove of both knees of ten sheep; defects of the right knees were filled with autologous PRP a...

Descripción completa

Detalles Bibliográficos
Autores principales: Carneiro, Márcio de Oliveira, Barbieri, Cláudio Henrique, Barbieri, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Ortopedia e Traumatologia Regional de São Paulo 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861962/
https://www.ncbi.nlm.nih.gov/pubmed/24453648
http://dx.doi.org/10.1590/S1413-78522013000200003
_version_ 1782295700825964544
author Carneiro, Márcio de Oliveira
Barbieri, Cláudio Henrique
Barbieri, José
author_facet Carneiro, Márcio de Oliveira
Barbieri, Cláudio Henrique
Barbieri, José
author_sort Carneiro, Márcio de Oliveira
collection PubMed
description OBJECTIVE: To assess the regeneration of osteochondral defects in the joint cartilage of the knee induced by autologous platelet-rich plasma (PRP). METHODS: Osteochondral defects produced in the trochlear groove of both knees of ten sheep; defects of the right knees were filled with autologous PRP and the left knees were left unfilled. Macroscopic and microscopic evaluation was carried out 12 week later. The results were evaluated by the total score of both macroscopic and microscopic evaluations comparing the two sides through the Wilcoxon paired test. RESULTS: Macroscopic appearance was not uniform among animals, nor was it different between the right and left knees (p=0.3125), and in no case the regenerated tissue was equal to the normal surrounding cartilage. At histological examination, apparently normal cartilage was not detected in any knee, but a poorly differentiated cartilage was present in 7 right knees, compared to 3 left knees. Fibrocartilaginous tissue was present in most of the remaining knees, with a significant difference in the overall score between right and left knees (p=0.0313). CONCLUSION: The PRP as used in this study has reparative properties of the joint cartilage of sheep knees, mostly by stimulating the formation of a fibrocartilaginous tissue. Laboratory investigation.
format Online
Article
Text
id pubmed-3861962
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Sociedade Brasileira de Ortopedia e Traumatologia Regional de São Paulo
record_format MEDLINE/PubMed
spelling pubmed-38619622014-01-22 Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps Carneiro, Márcio de Oliveira Barbieri, Cláudio Henrique Barbieri, José Acta Ortop Bras Original Article OBJECTIVE: To assess the regeneration of osteochondral defects in the joint cartilage of the knee induced by autologous platelet-rich plasma (PRP). METHODS: Osteochondral defects produced in the trochlear groove of both knees of ten sheep; defects of the right knees were filled with autologous PRP and the left knees were left unfilled. Macroscopic and microscopic evaluation was carried out 12 week later. The results were evaluated by the total score of both macroscopic and microscopic evaluations comparing the two sides through the Wilcoxon paired test. RESULTS: Macroscopic appearance was not uniform among animals, nor was it different between the right and left knees (p=0.3125), and in no case the regenerated tissue was equal to the normal surrounding cartilage. At histological examination, apparently normal cartilage was not detected in any knee, but a poorly differentiated cartilage was present in 7 right knees, compared to 3 left knees. Fibrocartilaginous tissue was present in most of the remaining knees, with a significant difference in the overall score between right and left knees (p=0.0313). CONCLUSION: The PRP as used in this study has reparative properties of the joint cartilage of sheep knees, mostly by stimulating the formation of a fibrocartilaginous tissue. Laboratory investigation. Sociedade Brasileira de Ortopedia e Traumatologia Regional de São Paulo 2013 /pmc/articles/PMC3861962/ /pubmed/24453648 http://dx.doi.org/10.1590/S1413-78522013000200003 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Carneiro, Márcio de Oliveira
Barbieri, Cláudio Henrique
Barbieri, José
Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
title Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
title_full Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
title_fullStr Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
title_full_unstemmed Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
title_short Platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
title_sort platelet-rich plasma gel promotes regeneration of articular cartilage in knees of sheeps
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861962/
https://www.ncbi.nlm.nih.gov/pubmed/24453648
http://dx.doi.org/10.1590/S1413-78522013000200003
work_keys_str_mv AT carneiromarciodeoliveira plateletrichplasmagelpromotesregenerationofarticularcartilageinkneesofsheeps
AT barbiericlaudiohenrique plateletrichplasmagelpromotesregenerationofarticularcartilageinkneesofsheeps
AT barbierijose plateletrichplasmagelpromotesregenerationofarticularcartilageinkneesofsheeps