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Activation of Drosophila hemocyte motility by the ecdysone hormone

Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise...

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Autores principales: Sampson, Christopher J., Amin, Unum, Couso, Juan-Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863427/
https://www.ncbi.nlm.nih.gov/pubmed/24285708
http://dx.doi.org/10.1242/bio.20136619
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author Sampson, Christopher J.
Amin, Unum
Couso, Juan-Pablo
author_facet Sampson, Christopher J.
Amin, Unum
Couso, Juan-Pablo
author_sort Sampson, Christopher J.
collection PubMed
description Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton.
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spelling pubmed-38634272013-12-16 Activation of Drosophila hemocyte motility by the ecdysone hormone Sampson, Christopher J. Amin, Unum Couso, Juan-Pablo Biol Open Research Article Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton. The Company of Biologists 2013-11-14 /pmc/articles/PMC3863427/ /pubmed/24285708 http://dx.doi.org/10.1242/bio.20136619 Text en © 2013. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Sampson, Christopher J.
Amin, Unum
Couso, Juan-Pablo
Activation of Drosophila hemocyte motility by the ecdysone hormone
title Activation of Drosophila hemocyte motility by the ecdysone hormone
title_full Activation of Drosophila hemocyte motility by the ecdysone hormone
title_fullStr Activation of Drosophila hemocyte motility by the ecdysone hormone
title_full_unstemmed Activation of Drosophila hemocyte motility by the ecdysone hormone
title_short Activation of Drosophila hemocyte motility by the ecdysone hormone
title_sort activation of drosophila hemocyte motility by the ecdysone hormone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863427/
https://www.ncbi.nlm.nih.gov/pubmed/24285708
http://dx.doi.org/10.1242/bio.20136619
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