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Activation of Drosophila hemocyte motility by the ecdysone hormone
Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863427/ https://www.ncbi.nlm.nih.gov/pubmed/24285708 http://dx.doi.org/10.1242/bio.20136619 |
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author | Sampson, Christopher J. Amin, Unum Couso, Juan-Pablo |
author_facet | Sampson, Christopher J. Amin, Unum Couso, Juan-Pablo |
author_sort | Sampson, Christopher J. |
collection | PubMed |
description | Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton. |
format | Online Article Text |
id | pubmed-3863427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-38634272013-12-16 Activation of Drosophila hemocyte motility by the ecdysone hormone Sampson, Christopher J. Amin, Unum Couso, Juan-Pablo Biol Open Research Article Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton. The Company of Biologists 2013-11-14 /pmc/articles/PMC3863427/ /pubmed/24285708 http://dx.doi.org/10.1242/bio.20136619 Text en © 2013. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Sampson, Christopher J. Amin, Unum Couso, Juan-Pablo Activation of Drosophila hemocyte motility by the ecdysone hormone |
title | Activation of Drosophila hemocyte motility by the ecdysone hormone |
title_full | Activation of Drosophila hemocyte motility by the ecdysone hormone |
title_fullStr | Activation of Drosophila hemocyte motility by the ecdysone hormone |
title_full_unstemmed | Activation of Drosophila hemocyte motility by the ecdysone hormone |
title_short | Activation of Drosophila hemocyte motility by the ecdysone hormone |
title_sort | activation of drosophila hemocyte motility by the ecdysone hormone |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863427/ https://www.ncbi.nlm.nih.gov/pubmed/24285708 http://dx.doi.org/10.1242/bio.20136619 |
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