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Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines

The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with cisplatin (3, 6, 13, and 18 μM) and sunitinib malate (1, 2, 4, 6, and 20 μM), either in isolation or combi...

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Autores principales: Arantes-Rodrigues, Regina, Pinto-Leite, Rosário, Fidalgo-Gonçalves, Lio, Palmeira, Carlos, Santos, Lúcio, Colaço, Aura, Oliveira, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863483/
https://www.ncbi.nlm.nih.gov/pubmed/24369536
http://dx.doi.org/10.1155/2013/791406
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author Arantes-Rodrigues, Regina
Pinto-Leite, Rosário
Fidalgo-Gonçalves, Lio
Palmeira, Carlos
Santos, Lúcio
Colaço, Aura
Oliveira, Paula
author_facet Arantes-Rodrigues, Regina
Pinto-Leite, Rosário
Fidalgo-Gonçalves, Lio
Palmeira, Carlos
Santos, Lúcio
Colaço, Aura
Oliveira, Paula
author_sort Arantes-Rodrigues, Regina
collection PubMed
description The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with cisplatin (3, 6, 13, and 18 μM) and sunitinib malate (1, 2, 4, 6, and 20 μM), either in isolation or combined, over the course of 72 hours. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. The combination index (CI) was calculated based on the Chou and Talalay method. In isolation, cisplatin and sunitinib malate statistically (P < 0.05) decrease cell viability in all cell lines in a dose-dependent manner, with the presence of autophagic vacuoles. A cell cycle arrest in early S-phase and in G(0)/G(1)-phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. Treatment of urinary bladder-cancer cells with a combination of cisplatin and sunitinib malate showed a synergistic effect (CI < 1). Autophagy and apoptosis studies showed a greater incidence when the combined treatment was put into use. This hints at the possibility of a new combined therapeutic approach. If confirmed in vivo, this conjugation may provide a means of new perspectives in muscle-invasive urinary bladder cancer treatment.
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spelling pubmed-38634832013-12-25 Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines Arantes-Rodrigues, Regina Pinto-Leite, Rosário Fidalgo-Gonçalves, Lio Palmeira, Carlos Santos, Lúcio Colaço, Aura Oliveira, Paula Biomed Res Int Research Article The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. Cells were treated with cisplatin (3, 6, 13, and 18 μM) and sunitinib malate (1, 2, 4, 6, and 20 μM), either in isolation or combined, over the course of 72 hours. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. The combination index (CI) was calculated based on the Chou and Talalay method. In isolation, cisplatin and sunitinib malate statistically (P < 0.05) decrease cell viability in all cell lines in a dose-dependent manner, with the presence of autophagic vacuoles. A cell cycle arrest in early S-phase and in G(0)/G(1)-phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. Treatment of urinary bladder-cancer cells with a combination of cisplatin and sunitinib malate showed a synergistic effect (CI < 1). Autophagy and apoptosis studies showed a greater incidence when the combined treatment was put into use. This hints at the possibility of a new combined therapeutic approach. If confirmed in vivo, this conjugation may provide a means of new perspectives in muscle-invasive urinary bladder cancer treatment. Hindawi Publishing Corporation 2013 2013-11-28 /pmc/articles/PMC3863483/ /pubmed/24369536 http://dx.doi.org/10.1155/2013/791406 Text en Copyright © 2013 Regina Arantes-Rodrigues et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arantes-Rodrigues, Regina
Pinto-Leite, Rosário
Fidalgo-Gonçalves, Lio
Palmeira, Carlos
Santos, Lúcio
Colaço, Aura
Oliveira, Paula
Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
title Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
title_full Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
title_fullStr Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
title_full_unstemmed Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
title_short Synergistic Effect between Cisplatin and Sunitinib Malate on Human Urinary Bladder-Cancer Cell Lines
title_sort synergistic effect between cisplatin and sunitinib malate on human urinary bladder-cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863483/
https://www.ncbi.nlm.nih.gov/pubmed/24369536
http://dx.doi.org/10.1155/2013/791406
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