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miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we e...

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Autores principales: Su, Xiaoping, Qian, Cheng, Zhang, Qian, Hou, Jin, Gu, Yan, Han, Yanmei, Chen, Yongjian, Jiang, Minghong, Cao, Xuetao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863901/
https://www.ncbi.nlm.nih.gov/pubmed/24309499
http://dx.doi.org/10.1038/ncomms3903
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author Su, Xiaoping
Qian, Cheng
Zhang, Qian
Hou, Jin
Gu, Yan
Han, Yanmei
Chen, Yongjian
Jiang, Minghong
Cao, Xuetao
author_facet Su, Xiaoping
Qian, Cheng
Zhang, Qian
Hou, Jin
Gu, Yan
Han, Yanmei
Chen, Yongjian
Jiang, Minghong
Cao, Xuetao
author_sort Su, Xiaoping
collection PubMed
description Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we examine miRNomes of mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and histone modification in HSCs and DCs at different differentiation stages. miR-30b, significantly upregulated via a TGF-beta/Smad3-mediated epigenetic pathway in regulatory DCs, can target Notch1 to promote IL-10 and NO production, suggesting that miR-30b is a negative regulator of immune response. We also identify miRNomes of in vivo counterparts of mature DCs and regulatory DCs and systematically compare them with DCs cultured in vitro. These results provide a resource for studying roles of miRNAs in stem cell biology, development and functional regulation of DC subsets.
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spelling pubmed-38639012013-12-20 miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Su, Xiaoping Qian, Cheng Zhang, Qian Hou, Jin Gu, Yan Han, Yanmei Chen, Yongjian Jiang, Minghong Cao, Xuetao Nat Commun Article Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we examine miRNomes of mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and histone modification in HSCs and DCs at different differentiation stages. miR-30b, significantly upregulated via a TGF-beta/Smad3-mediated epigenetic pathway in regulatory DCs, can target Notch1 to promote IL-10 and NO production, suggesting that miR-30b is a negative regulator of immune response. We also identify miRNomes of in vivo counterparts of mature DCs and regulatory DCs and systematically compare them with DCs cultured in vitro. These results provide a resource for studying roles of miRNAs in stem cell biology, development and functional regulation of DC subsets. Nature Pub. Group 2013-12-06 /pmc/articles/PMC3863901/ /pubmed/24309499 http://dx.doi.org/10.1038/ncomms3903 Text en Copyright © 2013, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Su, Xiaoping
Qian, Cheng
Zhang, Qian
Hou, Jin
Gu, Yan
Han, Yanmei
Chen, Yongjian
Jiang, Minghong
Cao, Xuetao
miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1
title miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1
title_full miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1
title_fullStr miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1
title_full_unstemmed miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1
title_short miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1
title_sort mirnomes of haematopoietic stem cells and dendritic cells identify mir-30b as a regulator of notch1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863901/
https://www.ncbi.nlm.nih.gov/pubmed/24309499
http://dx.doi.org/10.1038/ncomms3903
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