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Microbial whole‐cell arrays

The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essent...

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Detalles Bibliográficos
Autores principales: Elad, Tal, Lee, Jin Hyung, Belkin, Shimshon, Gu, Man Bock
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864447/
https://www.ncbi.nlm.nih.gov/pubmed/21261831
http://dx.doi.org/10.1111/j.1751-7915.2007.00021.x
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author Elad, Tal
Lee, Jin Hyung
Belkin, Shimshon
Gu, Man Bock
author_facet Elad, Tal
Lee, Jin Hyung
Belkin, Shimshon
Gu, Man Bock
author_sort Elad, Tal
collection PubMed
description The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high‐performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis and – most importantly – enhanced long‐term maintenance of viability and activity on the fabricated biochips.
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spelling pubmed-38644472014-02-12 Microbial whole‐cell arrays Elad, Tal Lee, Jin Hyung Belkin, Shimshon Gu, Man Bock Microb Biotechnol Reviews The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high‐performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis and – most importantly – enhanced long‐term maintenance of viability and activity on the fabricated biochips. Blackwell Publishing Ltd 2008-03 2008-01-11 /pmc/articles/PMC3864447/ /pubmed/21261831 http://dx.doi.org/10.1111/j.1751-7915.2007.00021.x Text en Copyright © 2008 The Authors. Journal compilation © 2007 Society for Applied Microbiology and Blackwell Publishing Ltd.
spellingShingle Reviews
Elad, Tal
Lee, Jin Hyung
Belkin, Shimshon
Gu, Man Bock
Microbial whole‐cell arrays
title Microbial whole‐cell arrays
title_full Microbial whole‐cell arrays
title_fullStr Microbial whole‐cell arrays
title_full_unstemmed Microbial whole‐cell arrays
title_short Microbial whole‐cell arrays
title_sort microbial whole‐cell arrays
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864447/
https://www.ncbi.nlm.nih.gov/pubmed/21261831
http://dx.doi.org/10.1111/j.1751-7915.2007.00021.x
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