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Microbial whole‐cell arrays
The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essent...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864447/ https://www.ncbi.nlm.nih.gov/pubmed/21261831 http://dx.doi.org/10.1111/j.1751-7915.2007.00021.x |
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author | Elad, Tal Lee, Jin Hyung Belkin, Shimshon Gu, Man Bock |
author_facet | Elad, Tal Lee, Jin Hyung Belkin, Shimshon Gu, Man Bock |
author_sort | Elad, Tal |
collection | PubMed |
description | The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high‐performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis and – most importantly – enhanced long‐term maintenance of viability and activity on the fabricated biochips. |
format | Online Article Text |
id | pubmed-3864447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38644472014-02-12 Microbial whole‐cell arrays Elad, Tal Lee, Jin Hyung Belkin, Shimshon Gu, Man Bock Microb Biotechnol Reviews The coming of age of whole‐cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines – the whole‐cell array. In the present review, we highlight the state‐of‐the‐art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high‐performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis and – most importantly – enhanced long‐term maintenance of viability and activity on the fabricated biochips. Blackwell Publishing Ltd 2008-03 2008-01-11 /pmc/articles/PMC3864447/ /pubmed/21261831 http://dx.doi.org/10.1111/j.1751-7915.2007.00021.x Text en Copyright © 2008 The Authors. Journal compilation © 2007 Society for Applied Microbiology and Blackwell Publishing Ltd. |
spellingShingle | Reviews Elad, Tal Lee, Jin Hyung Belkin, Shimshon Gu, Man Bock Microbial whole‐cell arrays |
title | Microbial whole‐cell arrays |
title_full | Microbial whole‐cell arrays |
title_fullStr | Microbial whole‐cell arrays |
title_full_unstemmed | Microbial whole‐cell arrays |
title_short | Microbial whole‐cell arrays |
title_sort | microbial whole‐cell arrays |
topic | Reviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864447/ https://www.ncbi.nlm.nih.gov/pubmed/21261831 http://dx.doi.org/10.1111/j.1751-7915.2007.00021.x |
work_keys_str_mv | AT eladtal microbialwholecellarrays AT leejinhyung microbialwholecellarrays AT belkinshimshon microbialwholecellarrays AT gumanbock microbialwholecellarrays |