Labeling Thiols on Proteins, Living Cells, and Tissues with Enhanced Emission Induced by FRET

Using N-(2-Aminoethyl)maleimide-cysteine(StBu) (Mal-Cys) as a medium, protein thiols were converted into N-terminal cysteines. After a biocompatible condensation reaction between the N-terminal cysteine and fluorescent probe 2-cyanobenzothiazole-Gly-Gly-Gly-fluorescein isothiocyanate (CBT-GGG-FITC),...

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Detalles Bibliográficos
Autores principales: Yuan, Yue, Wang, Xijun, Mei, Bin, Zhang, Dongxin, Tang, Anming, An, Linna, He, Xiaoxiao, Jiang, Jun, Liang, Gaolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3865488/
https://www.ncbi.nlm.nih.gov/pubmed/24343586
http://dx.doi.org/10.1038/srep03523

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3865488/
https://www.ncbi.nlm.nih.gov/pubmed/24343586
http://dx.doi.org/10.1038/srep03523

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