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Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome

Although insulin resistance in skeletal muscle is well-characterized, the role of circulating whole blood in the metabolic syndrome phenotype is not well understood. We set out to test the hypothesis that genes involved in inflammation, insulin signaling and mitochondrial function would be altered i...

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Autores principales: Tangen, Samantha E., Tsinajinnie, Darwin, Nuñez, Martha, Shaibi, Gabriel Q., Mandarino, Lawrence J., Coletta, Dawn K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866261/
https://www.ncbi.nlm.nih.gov/pubmed/24358323
http://dx.doi.org/10.1371/journal.pone.0084002
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author Tangen, Samantha E.
Tsinajinnie, Darwin
Nuñez, Martha
Shaibi, Gabriel Q.
Mandarino, Lawrence J.
Coletta, Dawn K.
author_facet Tangen, Samantha E.
Tsinajinnie, Darwin
Nuñez, Martha
Shaibi, Gabriel Q.
Mandarino, Lawrence J.
Coletta, Dawn K.
author_sort Tangen, Samantha E.
collection PubMed
description Although insulin resistance in skeletal muscle is well-characterized, the role of circulating whole blood in the metabolic syndrome phenotype is not well understood. We set out to test the hypothesis that genes involved in inflammation, insulin signaling and mitochondrial function would be altered in expression in the whole blood of individuals with metabolic syndrome. We further wanted to examine whether similar relationships that we have found previously in skeletal muscle exist in peripheral whole blood cells. All subjects (n=184) were Latino descent from the Arizona Insulin Resistance registry. Subjects were classified based on the metabolic syndrome phenotype according to the National Cholesterol Education Program’s Adult Treatment Panel III. Of the 184 Latino subjects in the study, 74 were classified with the metabolic syndrome and 110 were without. Whole blood gene expression profiling was performed using the Agilent 4x44K Whole Human Genome Microarray. Whole blood microarray analysis identified 1,432 probes that were altered in expression ≥1.2 fold and P<0.05 after Benjamini-Hochberg in the metabolic syndrome subjects. KEGG pathway analysis revealed significant enrichment for pathways including ribosome, oxidative phosphorylation and MAPK signaling (all Benjamini-Hochberg P<0.05). Whole blood mRNA expression changes observed in the microarray data were confirmed by quantitative RT-PCR. Transcription factor binding motif enrichment analysis revealed E2F1, ELK1, NF-kappaB, STAT1 and STAT3 significantly enriched after Bonferroni correction (all P<0.05). The results of the present study demonstrate that whole blood is a useful tissue for studying the metabolic syndrome and its underlying insulin resistance although the relationship between blood and skeletal muscle differs.
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spelling pubmed-38662612013-12-19 Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome Tangen, Samantha E. Tsinajinnie, Darwin Nuñez, Martha Shaibi, Gabriel Q. Mandarino, Lawrence J. Coletta, Dawn K. PLoS One Research Article Although insulin resistance in skeletal muscle is well-characterized, the role of circulating whole blood in the metabolic syndrome phenotype is not well understood. We set out to test the hypothesis that genes involved in inflammation, insulin signaling and mitochondrial function would be altered in expression in the whole blood of individuals with metabolic syndrome. We further wanted to examine whether similar relationships that we have found previously in skeletal muscle exist in peripheral whole blood cells. All subjects (n=184) were Latino descent from the Arizona Insulin Resistance registry. Subjects were classified based on the metabolic syndrome phenotype according to the National Cholesterol Education Program’s Adult Treatment Panel III. Of the 184 Latino subjects in the study, 74 were classified with the metabolic syndrome and 110 were without. Whole blood gene expression profiling was performed using the Agilent 4x44K Whole Human Genome Microarray. Whole blood microarray analysis identified 1,432 probes that were altered in expression ≥1.2 fold and P<0.05 after Benjamini-Hochberg in the metabolic syndrome subjects. KEGG pathway analysis revealed significant enrichment for pathways including ribosome, oxidative phosphorylation and MAPK signaling (all Benjamini-Hochberg P<0.05). Whole blood mRNA expression changes observed in the microarray data were confirmed by quantitative RT-PCR. Transcription factor binding motif enrichment analysis revealed E2F1, ELK1, NF-kappaB, STAT1 and STAT3 significantly enriched after Bonferroni correction (all P<0.05). The results of the present study demonstrate that whole blood is a useful tissue for studying the metabolic syndrome and its underlying insulin resistance although the relationship between blood and skeletal muscle differs. Public Library of Science 2013-12-17 /pmc/articles/PMC3866261/ /pubmed/24358323 http://dx.doi.org/10.1371/journal.pone.0084002 Text en © 2013 Tangen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tangen, Samantha E.
Tsinajinnie, Darwin
Nuñez, Martha
Shaibi, Gabriel Q.
Mandarino, Lawrence J.
Coletta, Dawn K.
Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome
title Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome
title_full Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome
title_fullStr Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome
title_full_unstemmed Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome
title_short Whole Blood Gene Expression Profiles in Insulin Resistant Latinos with the Metabolic Syndrome
title_sort whole blood gene expression profiles in insulin resistant latinos with the metabolic syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866261/
https://www.ncbi.nlm.nih.gov/pubmed/24358323
http://dx.doi.org/10.1371/journal.pone.0084002
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