A poor man’s BLASTX—high-throughput metagenomic protein database search using PAUDA

Summary: In the context of metagenomics, we introduce a new approach to protein database search called PAUDA, which runs ∼10 000 times faster than BLASTX, while achieving about one-third of the assignment rate of reads to KEGG orthology groups, and producing gene and taxon abundance profiles that ar...

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Detalles Bibliográficos
Autores principales: Huson, Daniel H., Xie, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Hitseq Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866550/
https://www.ncbi.nlm.nih.gov/pubmed/23658416
http://dx.doi.org/10.1093/bioinformatics/btt254
Descripción
Sumario:Summary: In the context of metagenomics, we introduce a new approach to protein database search called PAUDA, which runs ∼10 000 times faster than BLASTX, while achieving about one-third of the assignment rate of reads to KEGG orthology groups, and producing gene and taxon abundance profiles that are highly correlated to those obtained with BLASTX. PAUDA requires <80 CPU hours to analyze a dataset of 246 million Illumina DNA reads from permafrost soil for which a previous BLASTX analysis (on a subset of 176 million reads) reportedly required 800 000 CPU hours, leading to the same clustering of samples by functional profiles. Availability: PAUDA is freely available from: http://ab.inf.uni-tuebingen.de/software/pauda. Also supplementary method details are available from this website. Contact: daniel.huson@uni-tuebingen.de or xiechao@bic.nus.edu.sg

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