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Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells

This study was designed to investigate the anti-inflammatory effect of oyster shell extract on the production of pro-inflammatory factors [NO, reactive oxygen species (ROS), nuclear factor-kappa B (NF-κB), inducible nitric oxide synthase (iNOS) and cycloxygenase-2 (COX-2)] and pro-inflammatory cytok...

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Autores principales: Lee, Se-Young, Kim, Hak-Ju, Han, Ji-Sook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Food Science and Nutrition 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867150/
https://www.ncbi.nlm.nih.gov/pubmed/24471106
http://dx.doi.org/10.3746/pnf.2013.18.1.023
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author Lee, Se-Young
Kim, Hak-Ju
Han, Ji-Sook
author_facet Lee, Se-Young
Kim, Hak-Ju
Han, Ji-Sook
author_sort Lee, Se-Young
collection PubMed
description This study was designed to investigate the anti-inflammatory effect of oyster shell extract on the production of pro-inflammatory factors [NO, reactive oxygen species (ROS), nuclear factor-kappa B (NF-κB), inducible nitric oxide synthase (iNOS) and cycloxygenase-2 (COX-2)] and pro-inflammatory cytokines [Interleukin-1β (IL-1β), Interleukin-6 (IL-6) and TNF-α] in the lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. Cell viability, as measured by the MTT assay, showed that oyster shell extract had no significant cytotoxicity in Raw 264.7 cells. The treatment with oyster shell extract decreased the generation of intracellular reactive oxygen species dose dependently and increased antioxidant enzyme activities, such as SOD, catalase, GSH-px in LPS-stimulated macrophage cells. Oyster shell extract significantly suppressed the production of NO and also decreased the expressions of iNOS, COX-2 and NF-κB. Additionally, oyster shell extract significantly inhibited the production of IL-1β, IL-6, and TNF-α in LPS-stimulated Raw 264.7 cells. Thus, these results showed that the oyster shell extract had an anti-inflammatory effect on LPS-stimulated Raw 264.7 cells.
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spelling pubmed-38671502014-01-27 Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells Lee, Se-Young Kim, Hak-Ju Han, Ji-Sook Prev Nutr Food Sci Articles This study was designed to investigate the anti-inflammatory effect of oyster shell extract on the production of pro-inflammatory factors [NO, reactive oxygen species (ROS), nuclear factor-kappa B (NF-κB), inducible nitric oxide synthase (iNOS) and cycloxygenase-2 (COX-2)] and pro-inflammatory cytokines [Interleukin-1β (IL-1β), Interleukin-6 (IL-6) and TNF-α] in the lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. Cell viability, as measured by the MTT assay, showed that oyster shell extract had no significant cytotoxicity in Raw 264.7 cells. The treatment with oyster shell extract decreased the generation of intracellular reactive oxygen species dose dependently and increased antioxidant enzyme activities, such as SOD, catalase, GSH-px in LPS-stimulated macrophage cells. Oyster shell extract significantly suppressed the production of NO and also decreased the expressions of iNOS, COX-2 and NF-κB. Additionally, oyster shell extract significantly inhibited the production of IL-1β, IL-6, and TNF-α in LPS-stimulated Raw 264.7 cells. Thus, these results showed that the oyster shell extract had an anti-inflammatory effect on LPS-stimulated Raw 264.7 cells. The Korean Society of Food Science and Nutrition 2013-03 /pmc/articles/PMC3867150/ /pubmed/24471106 http://dx.doi.org/10.3746/pnf.2013.18.1.023 Text en Copyright © 2013 by The Korean Society of Food Science and Nutrition. All rights Reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Lee, Se-Young
Kim, Hak-Ju
Han, Ji-Sook
Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells
title Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells
title_full Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells
title_fullStr Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells
title_full_unstemmed Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells
title_short Anti-inflammatory Effect of Oyster Shell Extract in LPS-stimulated Raw 264.7 Cells
title_sort anti-inflammatory effect of oyster shell extract in lps-stimulated raw 264.7 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867150/
https://www.ncbi.nlm.nih.gov/pubmed/24471106
http://dx.doi.org/10.3746/pnf.2013.18.1.023
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