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Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups

PURPOSE: To investigate the possible free radical-scavenging activity of an extract of Cineraria maritima on selenite-induced cataractous lenses in Wistar rat pups. METHODS: In the present study, Wistar rat pups were divided into three experimental groups. On P10, Group I (control) rat pups received...

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Autores principales: Anitha, Thirugnanasambandhar Sivasubramanian, Muralidharan, Arumugam Ramachandran, Annadurai, Thangaraj, Jesudasan, Christdas Arul Nelson, Thomas, Philip Aloysius, Geraldine, Pitchairaj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867162/
https://www.ncbi.nlm.nih.gov/pubmed/24357923
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author Anitha, Thirugnanasambandhar Sivasubramanian
Muralidharan, Arumugam Ramachandran
Annadurai, Thangaraj
Jesudasan, Christdas Arul Nelson
Thomas, Philip Aloysius
Geraldine, Pitchairaj
author_facet Anitha, Thirugnanasambandhar Sivasubramanian
Muralidharan, Arumugam Ramachandran
Annadurai, Thangaraj
Jesudasan, Christdas Arul Nelson
Thomas, Philip Aloysius
Geraldine, Pitchairaj
author_sort Anitha, Thirugnanasambandhar Sivasubramanian
collection PubMed
description PURPOSE: To investigate the possible free radical-scavenging activity of an extract of Cineraria maritima on selenite-induced cataractous lenses in Wistar rat pups. METHODS: In the present study, Wistar rat pups were divided into three experimental groups. On P10, Group I (control) rat pups received an intraperitoneal injection of 0.89% saline. Rats in groups II (selenite-challenged, untreated) and III (selenite-challenged, C. maritima treated) received a subcutaneous injection of sodium selenite (19 μmol/kg bodyweight); Group III rat pups also received an intraperitoneal injection of the extract of C. maritima (350 mg/kg bodyweight) once daily P9–14. Both eyes of each pup were examined from P16 until P30. Cytochemical localization of nitroblue tetrazolium salts and generation of superoxide, hydroxyl, and nitric oxide levels were measured. The expression of the inducible nitric oxide synthase gene was evaluated with reverse transcription-PCR. Immunoblot analysis was also performed to confirm the differential expression of the inducible nitric oxide synthase protein. RESULTS: Subcutaneous injection of sodium selenite led to severe oxidative damage in the lenticular tissues, shown by increased formation of formazan crystals, elevated generation of superoxide, hydroxyl, and nitric oxide radicals, and elevated inducible nitric oxide synthase gene and protein expression that possibly contributed to the opacification of the lens and thus cataract formation. When rat pups were treated with intraperitoneal administration of the extract of C. maritima, the generation of free radicals as well as the messenger ribonucleic acid and protein expression of inducible nitric oxide synthase were maintained at near normal levels. CONCLUSIONS: The data generated by this study suggest that an ethanolic extract of C. maritima possibly prevents cataractogenesis in a rat model by minimizing free radical generation.
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spelling pubmed-38671622013-12-19 Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups Anitha, Thirugnanasambandhar Sivasubramanian Muralidharan, Arumugam Ramachandran Annadurai, Thangaraj Jesudasan, Christdas Arul Nelson Thomas, Philip Aloysius Geraldine, Pitchairaj Mol Vis Research Article PURPOSE: To investigate the possible free radical-scavenging activity of an extract of Cineraria maritima on selenite-induced cataractous lenses in Wistar rat pups. METHODS: In the present study, Wistar rat pups were divided into three experimental groups. On P10, Group I (control) rat pups received an intraperitoneal injection of 0.89% saline. Rats in groups II (selenite-challenged, untreated) and III (selenite-challenged, C. maritima treated) received a subcutaneous injection of sodium selenite (19 μmol/kg bodyweight); Group III rat pups also received an intraperitoneal injection of the extract of C. maritima (350 mg/kg bodyweight) once daily P9–14. Both eyes of each pup were examined from P16 until P30. Cytochemical localization of nitroblue tetrazolium salts and generation of superoxide, hydroxyl, and nitric oxide levels were measured. The expression of the inducible nitric oxide synthase gene was evaluated with reverse transcription-PCR. Immunoblot analysis was also performed to confirm the differential expression of the inducible nitric oxide synthase protein. RESULTS: Subcutaneous injection of sodium selenite led to severe oxidative damage in the lenticular tissues, shown by increased formation of formazan crystals, elevated generation of superoxide, hydroxyl, and nitric oxide radicals, and elevated inducible nitric oxide synthase gene and protein expression that possibly contributed to the opacification of the lens and thus cataract formation. When rat pups were treated with intraperitoneal administration of the extract of C. maritima, the generation of free radicals as well as the messenger ribonucleic acid and protein expression of inducible nitric oxide synthase were maintained at near normal levels. CONCLUSIONS: The data generated by this study suggest that an ethanolic extract of C. maritima possibly prevents cataractogenesis in a rat model by minimizing free radical generation. Molecular Vision 2013-12-16 /pmc/articles/PMC3867162/ /pubmed/24357923 Text en Copyright © 2013 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.
spellingShingle Research Article
Anitha, Thirugnanasambandhar Sivasubramanian
Muralidharan, Arumugam Ramachandran
Annadurai, Thangaraj
Jesudasan, Christdas Arul Nelson
Thomas, Philip Aloysius
Geraldine, Pitchairaj
Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups
title Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups
title_full Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups
title_fullStr Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups
title_full_unstemmed Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups
title_short Putative free radical-scavenging activity of an extract of Cineraria maritima in preventing selenite-induced cataractogenesis in Wistar rat pups
title_sort putative free radical-scavenging activity of an extract of cineraria maritima in preventing selenite-induced cataractogenesis in wistar rat pups
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867162/
https://www.ncbi.nlm.nih.gov/pubmed/24357923
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